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[(during)-288.9(incubation,)-287.9(it)-284.7(is)-290.1(recommended)-289.9(that)-288.1(cultures)-287.6(are)-287.8(incu-)]TJ 0 -1.1116 TD [(bated)-261.4(at)-262.7(the)-263.8(same)-261.2(environmental)-267.1(temperatures)-261(as)-262.4(the)-263.8(aquaria)]TJ T* [(from)-282(which)-284.1(samples)-281.7(were)-284(collected.)]TJ 7.3823 0 0 6.5614 205.5684 148.7055 Tm (16)Tj 9.843 0 0 9.843 72 133.3417 Tm [(While)-291.4(there)-290.2(are)-293.6(many)-289(recommendations)-300.1(online)-290.3(for)-291.2(zebra-)]TJ -1.0137 -1.1116 TD [(sh)-325.5(system)-331.3(disinfection)-326.9(postexposure)-332(to)-324.9(pathogens,)-326.6(there)-330.5(is)]TJ T* [(currently)-352.3(no)-342(step-by-step)-355.2(procedure)-347.4(available.)-352.5(In)-347.7(this)-344.3(study)]TJ 0 -1.1174 TD [(we)-286.5(describe)-282.9(a)-284.5(detailed)-283.4(protocol)-286.7(for)-285.5(the)-281.1(eradication)-287.8(of)-284.4(myco-)]TJ 0 -1.1116 TD [(bacteria)-284.4(which)-278.4(was)-281.6(used)-276.1(after)-284.4(a)-278.7(pathogen)-282(outbreak)-276.2(in)-278.8(a)-278.7(wild)]TJ T* [(zebrash)-259.6(colony)-261.3(at)-256.9(the)-258(University)-266.6(of)-255.6(Glasgow)-258.2(in)-261.5(2016/2017.)]TJ 0 -1.1174 TD [(A)-215.1(high)-217.6(bacterial)-216.5(load)-218.8(in)-209.7(the)-217.7(sh)-216.1(holding)-215.3(units,)-216.4(combined)-214.1(with)]TJ 0 -1.1116 TD [(high)-338.6(susceptibility)-340.7(to)-336.4(the)-338.7(disease,)-336.9(resulted)-339.5(in)-336.4(the)-338.7(loss)-338.2(of)-336.2(al-)]TJ 25.504 67.9184 TD [(most)-230.2(the)-229.2(entire)-228(sh)-221.8(colony.)-230.2(After)-226.5(a)-226.9(few)-229.9(months)-226.6(of)-226.8(attempting)]TJ 0 -1.1174 TD [(to)-457.3(treat)-463.1(the)-459.6(outbreak,)-458.2(the)-459.6(decision)-460.5(was)-460.2(made)-457.2(that)-460.8(the)-453.8(re-)]TJ 0 -1.1116 TD [(maining)-229.2(population)-225.7(would)-225.4(be)-228(culled.)-225.7(Before)-228.8(recolonizing)-230.2(the)]TJ T* [(system)-227.6(with)-230.2(new)-224.2(sh)-227.6(to)-221.2(continue)-230.3(with)-224.4(the)-229.2(project,)-226.8(the)-223.4(facility)]TJ T* [(had)-315.5(to)-307.6(be)-314.4(completely)-309.9(cleaned)-317.8(and)-309.7(the)-309.9(bacteria)-313.2(totally)-314.7(eradi-)]TJ 0 -1.1174 TD [(cated)-285.6(to)-278.8(prevent)-286.6(an)-279.8(outbreak)-281.9(from)-282(reoccurring.)]TJ 1.0137 -1.1116 TD [(Our)-240.3(aim)-242(is)-238.3(to)-238.5(provide)-245.1(a)-238.4(detailed)-243.1(disinfection)-246.2(procedure)-238(for)]TJ -1.0137 -1.1116 TD [(the)-332.9(effective)-331.5(elimination)-327.3(of)]TJ /F6 1 Tf 11.4791 0 TD [(M.)-322.4(haemophilum)]TJ /F5 1 Tf 7.1824 0 TD (and)Tj /F6 1 Tf 1.774 0 TD [(A.)-328.3(hydro-)]TJ -20.4354 -1.1174 TD (phila)Tj /F5 1 Tf 2.3096 0 TD [(from)-241.7(research-sta)-12.9(ndard)-242.8(zebrash)-248.1(units.)-245.2(The)-241.8(protocol)-246.4(in)]TJ -2.3096 -1.1116 TD [(this)-234.9(study)-235.8(was)-235.5(applied)-234.9(for)-233.6(two)-228.9(340)-170.3(L)-232.7(MBKI)-231.8(Aquatic)-235.8(Habitats)]TJ T* [(Z-hab)-359.2(stand-alone)-355.5(units,)-360.3(but)-349(this)-355.8(practice)-359.3(can)-357(be)-354.7(easily)-354.6(ap-)]TJ 0 -1.1174 TD [(plied)-197(at)-193.6(smaller)-195.6(or)-192.2(larger)-199.1(scales)-194.1(in)-192.4(sh)-193(facilities)-199.2(in)-192.4(the)-194.7(event)-192.3(of)]TJ 0 -1.1116 TD [(mycobacte)-10.2(rial)-190.1(infection.)-188.8(We)-189.9(also)-189.8(used)-189.7(the)-194.7(same)-186.3(protocol)-194.5(with)]TJ T* [(minor)-381.2(modications)-379.7(for)-377.6(the)-379(disinfection)-384.5(of)-376.5(six)-377.6(300)-164.6(L)-382.4(glass)]TJ T* [(aquarium)-398.4(large)-393.8(tank)-391.6(units.)-394.9(The)-397.3(protocol)-390.4(will)-397.3(be)-389.3(useful)-393.6(for)]TJ 0 -1.1174 TD [(disinfection)-545.7(process)-545.3(in)-543.7(zebrash)-547.6(facilities,)-542.5(especially)-545.7(for)]TJ 0 -1.1116 TD [(quarantine)-481.3(systems,)-485.4(where)-475.2(general)-483.6(cleanouts)-480(are)-477.9(required)]TJ T* [(because)-242.6(of)-232.6(the)-235(frequent)-235.8(repopulatio)-9(n)-231.5(with)-235.9(newly)-238.1(arrived)-234.7(sh.)]TJ /F4 1 Tf 8.9663 0 0 8.9663 313.0582 515.9054 Tm (Methods)Tj /F2 1 Tf 0 -1.8336 TD (Animals)Tj /F5 1 Tf 9.843 0 0 9.843 323.0361 483.0235 Tm [(In)-203.8(2015,)-197.9(a)-203.8(wild-origin)-197.8(population)-202.6(of)-203.8(zebrash)-202(was)-201(required)]TJ -1.0137 -1.1174 TD [(at)-256.9(the)-258(University)-255.1(of)-255.6(Glasgow)-258.2(to)-255.8(provide)-256.6(genetic)-259.1(diversity)-253.1(for)]TJ 0 -1.1116 TD [(an)-429.6(investigation)-437.6(of)-428.4(behavioral)-429.4(trait)-433.2(heritability,)-428.7(within)-434.1(the)]TJ T* [(Institute)-483.7(of)-486(Biodiversity)-482.2(Animal)-482.4(Health)-482.4(and)-482.5(Comparative)]TJ T* [(Medicine.)-293.4(Approximately)-289.8(2500)-286.6(wild)-287.8(zebrash)-288.4(were)-289.8(sourced)]TJ 0 -1.1174 TD [(from)-454.8(the)-453.8(Kosi)-454.5(River,)-454.7(India.)-447.9(They)-456(remained)-457.1(in)-451.6(quarantine)]TJ 0 -1.1116 TD [(holdings)-354.5(for)-354.6(2)-352.4(months)-359.1(and)-350(arrived)-355.7(at)-354.8(the)-355.9(facility)-357.2(at)-354.8(the)-350.2(be-)]TJ T* [(ginning)-307.5(of)-307.4(February)-310.4(2016.)-307.3(A)-307.2(subset)-307(of)-307.4(the)-304.1(original)-309.8(wild)-310.8(pa-)]TJ 0 -1.1174 TD [(rental)-366.3(generation)-366.1(was)-362.2(initially)-363.2(used)-362.5(for)-360.3(pilot)-357.2(studie)-7.8(s)-358(before)]TJ 0 -1.1116 TD [(breeding)-305(commenced.)-302.7(The)-293.6(eggs)-299.1(were)-301.3(surface)-304.7(sanitized)-301.6(with)]TJ T* [(bleach,)-370.8(following)-368.2(a)-365.1(standardize)-11(d)-364(zebrash)-369(egg)-367.3(disinfection)]TJ 0 -1.1174 TD [(protocol)-206(\(e.g.,)-204.8(5070)-166.8(ppm)-206.1(sodium)-203.6(hypochlorite)-206(for)-199.1(10)-169.2(min)]TJ 7.3823 0 0 6.5614 539.0361 366.8597 Tm (17)Tj 9.843 0 0 9.843 546.4628 362.4377 Tm (\),)Tj -23.7127 -1.1116 TD [(before)-314.1(the)-304.1(introduction)-308.6(to)-307.6(an)-308.6(independent)-309.6(recirculation)-313.2(zeb-)]TJ T* [(rash)-259.7(system)-256.4(for)-256.7(rearing.)-255.4(The)-259.1(F1)-249.7(offspring)-257.4(were)-261(produced)-253(in)]TJ ET 313.058 295.143 239.131 .22675 re f 313.058 68.372 239.131 .28348 re f 313.058 266.513 239.131 .22675 re f BT /F9 1 Tf 9.845 0 0 9.845 339.0235 310.7905 Tm (Table)Tj /F5 1 Tf 3.0002 0 TD (1.)Tj /F9 1 Tf 1.2496 0 TD [(Summary)-332(of)-337(the)-332.4(Water)-335.2(Quality)]TJ -6.6799 -1.0135 TD [(Parameters)-338.4(and)-332(Husbandry)-333.8(Routine)-338.5(in)-331.6(the)-332.4(Facility)]TJ /F6 1 Tf 10.9988 -2.8505 TD [(Z-hab)-333(units)]TJ 7.4688 1.0077 TD (Flow-through)Tj 1.2554 -1.0077 TD (systems)Tj /F5 1 Tf -19.9303 -1.9003 TD [(Water)-337.9(temperature)-1865.6(27.528.5)]TJ /F7 1 Tf 13.452 0 TD ()Tj /F5 1 Tf .3973 0 TD [(C)-4107.8(27.528.5)]TJ /F7 1 Tf 8.776 0 TD ()Tj /F5 1 Tf .3916 0 TD (C)Tj -23.0169 -1.0135 TD [(pH)-8237.5(7.07.5)-6167.6(6.87.3)]TJ 0 -1.0077 TD [(Conductivity)-4218.5(350400)]TJ /F12 1 Tf 13.118 0 TD (l)Tj /F5 1 Tf .5528 0 TD [(S)-4400.4(150200)]TJ /F12 1 Tf 8.6205 0 TD (l)Tj /F5 1 Tf .5528 0 TD (S)Tj -22.8441 -1.0135 TD (NO)Tj 7.3837 0 0 6.5625 327.2314 222.5196 Tm (2)Tj /F7 1 Tf 9.845 0 0 9.845 406.1479 224.1637 Tm (<)Tj /F5 1 Tf .5528 0 TD [(0.05)-161.8(mg/L)]TJ /F7 1 Tf 8.6148 0 TD (<)Tj /F5 1 Tf .5528 0 TD [(0.05)-167.6(mg/L)]TJ -19.176 -1.0135 TD (NO)Tj 7.3837 0 0 6.5625 327.2314 212.5417 Tm (3)Tj /F7 1 Tf 9.845 0 0 9.845 406.1479 214.1858 Tm (<)Tj /F5 1 Tf .5528 0 TD [(15)-163.2(mg/L)]TJ /F7 1 Tf 8.6148 0 TD (<)Tj /F5 1 Tf .5528 0 TD [(10)-163.2(mg/L)]TJ -19.176 -1.0135 TD (Ammonia)Tj /F7 1 Tf 9.4555 0 TD (<<)Tj /F5 1 Tf 1.0999 0 TD [(0.05)-167.6(mg/L)]TJ /F7 1 Tf 8.0677 0 TD (<<)Tj /F5 1 Tf 1.1056 0 TD [(0.05)-167.6(mg/L)]TJ -19.7288 -1.0077 TD [(Fish)-337.8(feeding)]TJ .9962 -1.0135 TD (regime)Tj /F7 1 Tf 3.1211 0 TD (>)Tj /F5 1 Tf .5528 0 TD [(4)-335(months)]TJ 4.7854 1.0135 TD (2)Tj /F7 1 Tf .6622 0 TD ()Tj /F5 1 Tf .7198 0 TD [(daily)-5776.5(2)]TJ /F7 1 Tf 8.4535 0 TD ()Tj /F5 1 Tf .7198 0 TD (daily)Tj -20.0109 -2.027 TD [(Fish)-337.8(feeding)]TJ /F7 1 Tf .9962 -1.0135 TD (<)Tj /F5 1 Tf .5528 0 TD [(4)-329.2(months)]TJ 7.9065 1.0135 TD (4)Tj /F7 1 Tf .6622 0 TD ()Tj /F5 1 Tf .7198 0 TD [(daily)-7889.9(ND)]TJ -10.8376 -2.0212 TD [(Tank)-340.3(cleaning)]TJ .9962 -1.0135 TD (regime)Tj T* (baby-juvenile)Tj /F7 1 Tf 8.4593 2.027 TD (<)Tj /F5 1 Tf .5528 0 TD [(1)-329.2(Month-daily;)]TJ /F7 1 Tf .4376 -1.0135 TD (>)Tj /F5 1 Tf .5528 0 TD [(1)-329.2(month-weekly)]TJ 9.7377 1.0135 TD (ND)Tj -20.7365 -3.0348 TD [(Tank)-340.3(cleaning)]TJ .9962 -1.0135 TD [(regime)-332.1(adults)]TJ 8.4593 1.0135 TD (Monthly)Tj .9905 -1.0135 TD [(\(change)-332.9(tanks\))]TJ 8.1771 1.0135 TD [(34)-331.2(Weekly)]TJ .9905 -1.0135 TD [(\(clean)-337.9(and)]TJ T* (siphon\))Tj -19.6136 -1.0135 TD [(Adult)-334.4(sh)-336.6(holding)]TJ .9962 -1.0077 TD (density)Tj 8.4593 1.0077 TD [(34)-331.2(sh/L)-5004.4(0.51.5)-334.2(sh/L)]TJ -9.4555 -2.0212 TD [(Light)-334.7(cycle)-4822.8(13)-163.2(h)-335(L:11)-166.5(h)-335(D)-3554.8(13)-169(h)-329.2(L:11)-166.5(h)-335(D)]TJ 8.7511 0 0 8.7511 321.0519 56.5795 Tm [(ND,)-335.3(no)-328.1(data)-339.8(present.)]TJ /F4 1 Tf 8.9663 0 0 8.9663 62.022 749.6502 Tm (78)Tj 40.846 0 TD (RA)Tj .9168 .2087 TD ()Tj .5185 -.2087 TD [(CZ,)-334.5(DWYER,)-341.5(AND)-330.9(KILLEN)]TJ ET endstream endobj 49 0 obj <>stream /GS1 gs BT /F5 1 Tf 9.843 0 0 9.843 59.7543 725.1588 Tm 0 0 0 rg 0 Tc 0 Tw [(May/June)-392.1(2016)-384.5(and)-384.6(were)-387.7(reared)-390.2(for)-389.1(use)-384.4(in)-382.5(behavioral)-389.1(and)]TJ 0 -1.1174 TD [(selection)-480.1(experiments.)-481.1(The)-478(F1)-474.3(generation)-481.3(developed)-480.1(well)]TJ 0 -1.1116 TD [(with)-310.8(no)-301.7(initial)-309(health)-302.9(issues)-306.8(arising.)-311.8(Holding)-302.6(and)-304(husbandry)]TJ T* [(conditions)-225.5(can)-224.5(be)-222.2(seen)-219.6(in)-221.2(Table)-228(1.)-217.6(The)-224.5(sh)-221.8(were)-226.4(to)-221.2(be)-222.2(used)-218.5(in)]TJ T* [(behavioral)-216.3(and)-206.1(heritability)-212.1(studies)-210.3(for)-210.6(which)-209.3(they)-207.3(would)-213.9(need)]TJ 0 -1.1174 TD [(to)-491.9(be)-498.7(individually)-493.1(identied)-496.3(\(both)-489.4(parental)-496.3(and)-494(offspring)]TJ 0 -1.1116 TD [(population\))-7.9(.)-423.9(T)0(o)-429.6(make)-428.4(identication)-427.2(easier,)-433.9(it)-428.7(was)-425.6(decided)]TJ T* [(that)-345.6(the)-344.4(sh)-337(would)-346.3(be)-343.2(tagged)-343.1(with)-339.6(four)-344.2(color)-340.8(combinations)]TJ 0 -1.1174 TD [(on)-405.4(their)-404.3(dorsal)-405.1(side)-408.7(using)-402.9(visible)-406.5(implant)-402.2(elastomer)-412.1(\(VIE\))]TJ 0 -1.1116 TD [(tags)-293.5(from)-287.8(Northwest)-295.1(Marine)-292.2(Technology,)-288.8(Inc.)-290.1(\(Fig.)-290(1\).)-293.6(This)]TJ T* [(method)-400.8(involved)-396.1(placing)-402(the)-396.3(sh)-400.4(under)-398.3(anesthesia)-401.4(and)-396.1(in-)]TJ 0 -1.1174 TD [(jecting)-430.9(colored)-424.8(ink)-423.9(under)-421.3(the)-425.1(skin,)-428.2(which)-422.4(results)-429.2(in)-422.8(small)]TJ 0 -1.1116 TD [(wounds)-306.8(at)-308.8(the)-304.1(injection)-312.3(sites.)-303.7(Visible)-309.4(elastomer)-314.2(tagging)-302.9(is)-307.4(a)]TJ T* [(routine)-291.3(identicati)-10.3(on)-284.4(method)-297.1(that)-288.1(is)-290.1(particularly)-293.6(common)-292.5(in)]TJ T* [(sh)-314(studies,)-311.7(including)-311(small-sized)-310.5(sh.)]TJ 7.3823 0 0 6.5614 214.8661 576.1132 Tm (18)Tj 9.843 0 0 9.843 225.2976 571.7479 Tm [(The)-310.9(F1)-313.1(generation)]TJ -16.8183 -1.1174 TD [(of)-336.2(sh)-331.2(was)-333.4(tagged)-331.6(in)-330.6(late)-335.3(November)-335.8(2016)-326.9(following)-333.7(a)-330.6(VIE)]TJ 0 -1.1116 TD [(tagging)-516(process)-510.7(where)-515.5(needles)-512.2(were)-514.4(sterilized)-515.9(with)-512.4(70%)]TJ T* 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[(neath)-427.8(the)-424.7(skin.)-427.7(Visible)-423.9(are)-425.7(two)-430.1(colored)-424.1(marks)-427.5(\()]TJ /F6 1 Tf 20.1376 0 TD (green)Tj /F5 1 Tf 2.7008 0 TD (and)Tj /F6 1 Tf -22.8384 -1.0077 TD (red)Tj /F5 1 Tf 1.336 0 TD [(\))-283.2(o)0(n)-278.4(the)-286.5(dorsal)-283.5(side.)-284.9(Photo)-281.4(credit:)-280.5(A.)-287.4(Ra)]TJ 15.7323 .0403 TD ()Tj .3858 -.0403 TD [(cz.)-284.1(Color)-282.5(images)]TJ -17.4541 -1.0135 TD [(are)-339.3(available)-336.6(online.)]TJ /F4 1 Tf 8.9663 0 0 8.9663 59.7543 749.7069 Tm [(ERADICATION)-340.4(OF)]TJ /F13 1 Tf 8.9975 0 TD (M)Tj 6.4556 0 0 6.4556 147.9118 749.7069 Tm [(YCOBACTER)-14.6(IUM)]TJ /F4 1 Tf 8.9663 0 0 8.9663 540.0565 749.7069 Tm (79)Tj ET endstream endobj 51 0 obj <>stream Adobed       U  s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjzm!1AQa"q2#BRbr3$4CS%cs5DT &6E'dtU7()󄔤euFVfvGWgw8HXhx9IYiy*:JZjz?`'! :A@}ڌz3 j F|p&|+j_jp=)AM}rƓEQDIfE)6mQQn:}\kU$no9U#nجl%.*TodcZɑeZJ\wBSZ};e_ꅁ翨E0}!ܹ$)2?1ĩ&_'eEJ:FٷɳƢ(ް<\(N0 <.? 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w?[FXu5Zi=9بϮ9j-[SJe^܋[,GX\\KoKVʲC/)˽F9t~-M~^)5EӷYGC/)|j<[-q5?0i5ؓ.F+HmڦؓSYg?i5iݍ)giU1)ؼimIfcNy!]Q`uYiy(Vnmym$;y[v':䖽$gWt״IZb۟݁h4IU32\4-;;(SSQLǏ捝=sCUɎ[=|Upcߓ!NqRḱ)بM;\Ȥ#}~;[,9}soGO9 vvS)Q֟3+g`YF THcɯǛ=uU8:Z}-zz#KzC> '7b2GS{_W-!}zq٘gi=eIr%j-#b_ endstream endobj 56 0 obj <>stream /GS1 gs BT /F5 1 Tf 9.843 0 0 9.843 62.022 418.3369 Tm 0 0 0 rg 0 Tc 0 Tw [(mortality)-185.6(and)-183(persistent)-186.1(symptoms)-189.6(within)-180.7(the)-183.1(population)-185.4(such)]TJ 0 -1.1174 TD [(as)-343(raised)-348.7(scales,)-341.6(ulcers,)-346.3(ascites,)-348.6(and)-344.3(hemorrhage.)-347.4(After)-341.7(his-)]TJ 0 -1.1116 TD [(tology)-329.4(reports)-331.3(of)-324.7(different)-334.9(agents)-331.4(\(including)-329.3(a)-330.6(possible)]TJ /F6 1 Tf 22.676 0 TD (My-)Tj -22.676 -1.1116 TD (cobacterium)Tj /F5 1 Tf 5.3392 0 TD [(species)-333.5(presence\),)-334.5(as)-331.5(a)-324.8(precaution)-337.3(the)-327.1(parental)]TJ -5.3392 -1.1174 TD 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){2-v=yeWL9,:֎˩eln̡^iu^L?6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ث endstream endobj 62 0 obj <>stream /GS1 gs BT /F5 1 Tf 9.843 0 0 9.843 59.7543 725.1588 Tm 0 0 0 rg 0 Tc 0 Tw [(displaying)-214(clinical)-214.4(signs)-212.6(for)-210.6(histopathological)-212.9(examination)-213.1(to)]TJ 0 -1.1174 TD [(the)-281.1(Institute)-287.8(of)-278.6(Aquaculture,)-285.1(University)-283.9(of)-278.6(ż.)]TJ 1.0137 -1.1116 TD [(In)-411.1(the)-413.5(F1)-411(population,)-413.4(clinical)-410.2(signs)-414.2(of)-411.1(disease)-414.1(began)-411(to)]TJ -1.0137 -1.1116 TD [(occur)-364.9(in)-359.4(early)-365(2017.)-359.2(An)-360.2(in-house)-367(investigation)-362.7(process)-361(en-)]TJ T* [(sued.)-239.2(Two)-235.8(biolm)-238.3(and)-240.6(sh)-233.3(lesion)-238.2(swab)-236.6(samples)-241.4(were)-238(stained)]TJ 0 -1.1174 TD [(with)-466.3(ZN)-465(acid-fast)-468.4(stain.)-459.4(Results)-470.6(revealed)-462.8(acid-fast)-468.4(bacilli)]TJ 0 -1.1116 TD 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[(rmation)-316.5(of)-313.2(death)-8(.)-308.7(These)-319.9(sh)-314(were)-318.6(immediately)-318.1(transferred)]TJ T* [(to)-394(sterile)-401.9(5)-162.4(m)0(L)-396.3(microtubes)-400.5(and)-396.1(appropriately)-398.3(labelled)-398.6(with)]TJ 0 -1.1174 TD [(their)-185.4(elastomer)-187.5(identicati)-10.3(on)-180.7(tag)-183.1(and)-183(date.)-188.8(Two)-183.9(biolm)-186.5(swabs)]TJ 0 -1.1116 TD [(were)-376.2(taken)-370.8(from)-368.4(each)-370.8(Z-hab)-370.7(unit.)-371(Biolm)-371.8(swab)-369.1(sticks)-370.5(and)]TJ T* [(instructions)-417.7(were)-422.3(provided)-413.3(by)-416.9(Charles)-419.9(River)-416.7(Laboratories.)]TJ T* [(The)-316.7(swabs)-318.2(were)-312.8(collected)-320.2(from)-316.6(the)-315.6(part)-310.9(of)-313.2(the)-315.6(recirculation)]TJ 0 -1.1174 TD [(unit)-281.1(that)-282.3(displayed)-283(the)-275.3(largest)-286.5(buildup)-278.7(of)-278.6(organic)-280.8(matter,)-278.8(the)]TJ 0 -1.1116 TD [(spillway)-431.5(container)-434.1(\(backow)-426.5(efuent)-430.4(water)-429.3(collector)-434.2(con-)]TJ T* [(tainer)-418.1(that)-414.8(collects)-415.7(the)-413.5(outow)-416.4(water)-417.7(from)-414.5(the)-413.5(whole)-416.6(unit)]TJ 0 -1.1174 TD [(before)-210.5(entering)-213(back)-208.3(into)-206.2(the)-206.2(sump\).)-211.5(The)-207.2(swab)-207.8(collection)-210.9(was)]TJ 0 -1.1116 TD [(concentrated)-336(at)-331.8(the)-332.9(airwater)-337(interface)-337.3(of)-330.5(the)-332.9(spillway)-333.6(con-)]TJ T* [(tainer)-285.6(\(Fig.)-278.5(2\).)]TJ 1.0137 -1.1174 TD .0072 Tc [(A)-6.3(f).4(t)-5.5(e)-.8(r)-529.5(t)-5.5(h)-5.4(e)-525(h)-5.4(i)-5.5(st)-5.5(ol)-5.5(o)-5.4(g).3(y)-529.5(r)-5.4(ep)-5.4(or)-5.4(t)-529.7(c)-6.6(on)-5.4(r)-5.4(me)-6.6(d)-529.5(h).3(i)-5.5(s)-5.2(to)-5.4(lo)-5.4(gi)-5.5(ca)-6.6(l)-529.7(a)-.8(n)-5.4(d)]TJ -1.0137 -1.1116 TD .0119 Tc 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[(spp.)-403.2(PC)-4.4(R)-401.9(P)-3.3(anel)-401(scre)-4.6(ening)-400.8(t)-3.5(ha)-4.6(t)-401(t)2.2(e)-4.6(s)-3.2(ts)-406.4(ea)-4.6(ch)]TJ -6.8253 -1.1116 TD .0116 Tc [(sampl)4.6(e)-319(f)4.8(o)-1(r)-317.8(t)4.6(he)-313.2(si)4.6(x)-317.8(m)3.5(ost)-312.2(c)-2.2(o)4.7(m)-2.2(mon)-312(s)-.8(peci)4.6(es)-311.8(of)-317.8(mycob)4.7(a)-2.2(cteria:)]TJ /F6 1 Tf 0 -1.1174 TD .0108 Tc [(M)-2.9(.)-297.9(chelonae)]TJ /F5 1 Tf 5.1089 0 TD 0 Tc (,)Tj /F6 1 Tf .5587 0 TD .013 Tc [(M.)-289.9(for)6.3(t).3(u)6.1(itu)6.1(m)]TJ /F5 1 Tf 5.2241 0 TD 0 Tc (,)Tj /F6 1 Tf .5587 0 TD .0115 Tc [(M.)-297.2(ha)4.6(emop)4.6(hi)4.5(lum)]TJ /F5 1 Tf 6.9577 0 TD 0 Tc (,)Tj /F6 1 Tf .5587 0 TD .0134 Tc [(M.)-289.5(mar)6.7(i).7(n)6.5(u).8(m)]TJ /F5 1 Tf 5.0743 0 TD 0 Tc (,)Tj /F6 1 Tf -24.041 -1.1116 TD .0124 Tc [(M.)-365.4(p)5.5(e)-1.4(regr)5.7(in)5.5(um)]TJ /F5 1 Tf 6.2435 0 TD .3778 Tc [(,a)369.8(n)365.2(d)]TJ /F6 1 Tf 2.4709 0 TD .0113 Tc [(M.)-366.5(abs)4.6(cessu)4.4(s)]TJ /F5 1 Tf 5.5005 0 TD .0133 Tc [(.)-364.5(T)5.3(he)-363.3(co)6.4(mp)6.4(an)6.4(y)-367.9(u)6.4(se)5.3(s)-367.7(t).6(h)6.4(e)]TJ -14.215 -1.1116 TD .0072 Tc [(h)-5.4(i)-5.5(gh)-5.4(ly)-402.8(s)-5.2(e)-6.6(ns)-5.2(it)-5.5(iv)-5.4(e)-404(T)-6.6(aq)-5.4(Ma)-6.6(n)]TJ /F7 1 Tf 7.3823 0 0 6.5614 162.9354 126.7086 Tm 0 Tc ()Tj /F5 1 Tf 9.843 0 0 9.843 172.8566 122.3433 Tm .0116 Tc [(RT-)4.8(P)-.9(CR)-399.5(met)4.6(h)-1(o)4.7(d)-398.4(to)-398.4(detect)-398.6(ge-)]TJ -11.4906 -1.1174 TD [(netic)-336.2(material)-341(fr)4.8(om)-336.3(con)4.7(t)-1.1(ami)4.6(n)-1(ated)-340.8(ag)4.7(en)4.7(ts,)-337.4(g)-1(r)4.8(e)-2.2(atl)4.6(y)-340.8(redu)4.7(cin)4.7(g)]TJ 0 -1.1116 TD .0071 Tc [(t)-5.6(h)-5.5(e)-502(n)-5.5(um)-6.7(be)-6.7(r)-506.6(o).2(f)-506.6(f)-5.5(al)-5.6(se)-507.8(p)-5.5(o).2(s)-5.3(i)-5.6(ti)-5.6(ve)-6.7(s.)-508.9(Ap)-5.5(pr)-5.5(o)-5.5(p).2(r)-5.5(i).1(a)-6.7(t).1(e)-507.8(p)-5.5(os)-5.3(i)-5.6(t).1(i)-5.6(v).2(e)-507.8(a)-6.7(nd)]TJ T* .0118 Tc [(negat)4.8(i)-.9(v)4.9(e)-278.4(co)4.9(nt)4.8(rol)4.8(s)-277.1(w)-1.7(ere)-278.4(u)-.8(s)5.1(e)-2(d)-277.3(a)3.8(s)-282.8(p)4.9(er)-277.2(company)-277.3(p)-.8(r)5(o)-.8(t)4.8(o)-.8(cols)5.1(.)-279.6(F)5.1(or)]TJ 0 -1.1174 TD [(sampl)4.8(e)-261.2(p)4.9(r)-.8(eparat)4.8(io)4.9(n,)-256.6(th)4.9(e)-261.2(c)3.8(ompany)-260(g)4.9(r)-.8(o)4.9(u)-.8(n)4.9(d)-260(an)4.9(d)-260(h)4.9(omog)4.9(en)4.9(ized)]TJ 0 -1.1116 TD .0123 Tc [(the)-306.7(w)-1.2(h)5.4(o)-.3(l)5.3(e)-312.5(b)5.4(ody)-305.6(of)-305.5(th)5.4(e)-312.5()-.2(s)5.6(h)-311.3(i)5.3(n)-311.3(bu)5.4(ff)5.5(er)-311.3(and)-305.6(u)-.3(s)5.6(e)-1.5(d)-311.3(t)5.3(hi)5.3(s)-311.1(f)-.3(o)5.4(r)-311.3(t)5.3(o)-.3(tal)]TJ T* .0121 Tc [(nucleic)-278.1(aci)5.1(d)-282.7(p)5.2(u)-.5(r)5.3(i)-.6()5.4(cat)5.1(io)5.2(n.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 310.8472 725.7825 Tm 0 Tc [(System)-335.3(disinfection)-335.3(protocol)]TJ /F5 1 Tf 9.843 0 0 9.843 320.8251 709.3416 Tm 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.9964 -1.7221 TD [(\(1\))-504.3(Soak)-299.1(a)-13.8(l)-7(l)-312.3(t)-12.7(a)-8(n)-12.6(k)-6.9(s)-12.4(,)-314.4(b)-6.9(a)-13.8(f)-6.8()-12.5(e)-8(s)-12.4(,)-308.7(a)-13.8(n)-12.6(d)-306.4(l)-12.7(i)-7(d)-12.6(s)-311.9(i)-12.7(n)-306.4(b)-12.6(l)-7(e)-13.8(a)-13.8(c)-8(h)-312.1(\()-6.8(m)-13.8(i)-7(n)-12.6(i)-12.7(m)-8.1(u)-12.6(m)]TJ 1.6703 -1.1116 TD .0122 Tc [(concen)5.3(tr)5.4(ati)5.2(o)-.4(n)-513(2)5.3(00)5.3(2)5.3(50)-167.4(p)5.3(p)-.4(m\))-513(fo)5.3(r)-513(2)-167.4(h)-513(b)5.3(ef)5.4(ore)-508.4(scr)5.4(u)-.4(b)5.3(-)]TJ 0 -1.1174 TD .0112 Tc [(b)4.3(i)-1.5(ng)-335.5(and)-335.5(r)-1.4(i)4.2(n)-1.4(s)4.5(i)-1.5(ng)-335.5(wit)4.2(h)-341.2(h)4.3(o)-1.4(t)-335.6(w)3.5(ater)-335.4(\()]TJ /F11 1 Tf 13.9327 0 TD 0 Tc (*)Tj /F5 1 Tf 1.0079 0 TD .0126 Tc (50)Tj /F7 1 Tf 1.0195 0 TD 0 Tc ()Tj /F5 1 Tf .4089 0 TD .0115 Tc [(C\).)-337.5(A)3.8(ll)-335.3(part)4.5(s)]TJ -16.3691 -1.1116 TD .0072 Tc [(s)-5.2(h).3(o)-5.4(u).3(l)-5.5(d)-345.2(be)-346.4(a)-6.6(i)-5.5(r-)-5.4(dr)-5.4(i)-5.5(e)-.8(d)-345.2(b)-5.4(ef)-5.4(or)-5.4(e)-346.4(r)-5.4(eu)-5.4(s)-5.2(e)-.8(.)-347.6(M)-.6(o)-5.4(r).4(e)-346.4(t)-5.5(h)-5.4(a)-.8(n)-345.2(o)-5.4(ne)-346.4(s)-5.2(e)-.8(t)]TJ T* .0073 Tc [(of)-564(ta)-6.5(nk)-5.3(s)-558(a)-6.5(nd)-558.2(m)-6.5(a)-.7(t)-5.4(e)-.7(r)-5.3(i)-5.4(al)-5.4(s)-558(f).5(o)-5.3(r)-558.2(e)-6.5(a)-.7(c)-6.5(h)-558.2(un)-5.3(i)-5.4(t)-558.4(ar)-5.3(e)-559.4(u).4(s)-5.1(u)-5.3(al)-5.4(ly)]TJ T* .0131 Tc [(p)6.2(r).5(es)6.4(en)6.2(t)-270.3(i).4(n)-264.5(r).5(e)5.1(s).7(ea)5.1(rc)5.1(h)-270.2(f).5(a)5.1(c)-.7(i)6.1(l).4(i)6.1(tie)5.1(s.)-266.8(Th)6.2(er)6.3(ef)6.3(ore)5.1(,)-266.8(i).3(t)-270.4(i)6.1(s)-270(a)5.1(dvi)6.1(se)5.1(d)]TJ 0 -1.1174 TD .0113 Tc [(t)4.3(o)-249(clean)-243.2(a)-2.5(n)4.4(d)-243.2(dry)-243.2(all)-243.4(t)-1.4(anks)-243(th)4.4(or)4.5(oug)4.4(hl)4.3(y)-249(s)4.6(o)-243.2(t)-1.4(hat)-243.4(t)-1.4(hey)-243.2(c)-2.5(an)]TJ 0 -1.1116 TD .0116 Tc [(b)4.7(e)-342(remo)4.7(ved)-335.1(a)-2.2(nd)-340.8(s)4.9(t)-1.1(o)4.7(r)-1(ed)-335.1(els)4.9(e)-2.2(where)-342(b)-1(efo)4.7(r)-1(e)-342(c)3.6(ommenc-)]TJ T* .0119 Tc [(i)4.9(n)-.7(g)-386.6(r)-.7(o)5(o)-.7(m)-387.8(d)-.7(i)4.9(s)-.5(in)5(fect)4.9(ion)5(.)-388.9(Pl)4.9(ease)-387.8(no)5(te)-387.8(th)5(at)-386.7(many)-386.6(com-)]TJ 0 -1.1174 TD .0069 Tc [(pa)-6.9(n)-5.7(i)-.1(e)-6.9(s)-783.1(su)-5.7(pp)-5.7(l)-5.8(y)-777.5(z)-6.9(e)-1.1(b)-5.7(r)-5.7(a)-1.1()-5.5(s)-5.5(h)-777.5(r)-5.7(e)-1.1(c)-6.9(i)-5.8(rc)-6.9(ul)-5.8(at)-5.8(i)-5.8(o)0(n)-783.3(u)-5.7(ni)-5.8(ts)-783.1(a)-6.9(n)0(d)]TJ 0 -1.1116 TD .0114 Tc [(h)4.5(a)-2.4(ve)-261.6(man)4.5(ufact)4.4(ured)-266.2(t)4.4(an)4.5(ks)-260.2(that)-266.3(can)-260.4(b)-1.2(e)-261.6(c)-2.4(l)4.4(e)-2.4(aned)-266.2(i)4.4(n)-266.2(cage)]TJ T* .0098 Tc [(wa)-4(she)-4(r)3(s.)-437.1(Some)-441.7(companie)-4(s)-434.6(d)-2.8(esign)-434.8(t)-2.9(anks)-434.6(which)-434.8(c)-4(an)]TJ 0 -1.1174 TD .0131 Tc [(a)5.1(l).4(so)-379.7(be)-380.8(au)6.2(to)6.2(cla)5.1(v).5(e)5.1(d)-385.4(b)6.2(u).5(t)-379.8(d).5(u)6.2(e)-386.6(t)6.1(o)-385.4(th)6.2(ei)6.1(r)-385.4(l)6.1(arg)6.2(e)-386.6(s)6.4(i).4(z)5.1(e)-386.6(\()6.3(3)-166.5(L)5(;)]TJ 0 -1.1116 TD .0112 Tc [(1)4.3(0)-168.4(L\))-306.6(thi)4.2(s)-306.5(is)-306.5(o)4.3(f)-1.4(ten)-306.7(n)-1.4(o)4.3(t)-306.8(feasi)4.2(b)]TJ 11.7498 0 TD .0116 Tc [(l)4.6(e)-2.2(,)-302.8(e)-2.2(sp)4.7(ecially)-306.3(i)4.6(n)-306.3(smal)4.6(ler)]TJ -11.7498 -1.1116 TD .0119 Tc [(f)5.1(aci)4.9(li)4.9(ties.)-360.1(I)5.1(t)-358(is)-357.6(al)4.9(so)-357.8(go)5(od)-357.8(pr)5.1(actice)-359(t)-.8(o)-357.8(c)-1.9(l)4.9(e)-1.9(an)-363.6(con)5(t)-.8(ami)4.9(-)]TJ T* .0109 Tc [(n)4(a)-2.9(ted)-266.7(s)-1.5(y)4(s)-1.5(t)3.9(e)-2.9(ms)-266.5(such)-266.7(as)-266.5(quarant)3.9(ine)-262.1(uni)3.9(ts)-266.5(s)4.2(e)-2.9(p)4(a)-2.9(rately)4(,)-269(t)3.9(o)]TJ 0 -1.1174 TD .0116 Tc [(avoi)4.6(d)-358.1(c)-2.2(o)4.7(n)-1(t)4.6(a)-2.2(minati)4.6(on)-358.1(t)4.6(o)-358.1(the)-353.5(m)-2.2(ain)-358.1(f)-1(aci)4.6(l)-1.1(i)4.6(t)-1.2(y;)-358.2(t)4.6(h)-1(erefor)4.8(e,)]TJ 0 -1.1116 TD .0072 Tc [(ma)-6.6(nu)-5.4(al)-575.8(t)-5.5(a)-6.6(nk)-575.6(c)-6.6(l).2(e)-6.6(a)-.8(n)-5.4(i)-5.5(ng)-575.6(s)-5.2(h).3(o)-5.4(u)-5.4(l).2(d)-575.6(b)-5.4(e)-576.8(i).2(n)-5.4(v).3(o)-5.4(l)-5.5(ve)-6.6(d)-575.6(i).2(n)-575.6(t)-5.5(he)]TJ T* .0127 Tc [(d)5.8(i)0(si)5.7(nf)5.9(ect)5.7(io)5.8(n)-334(p)5.8(roces)6(s.)]TJ -1.6703 -1.1174 TD 0 Tc [(\(2\))-504.3(Day)-299.1(1set)-301.5(system)-302.5(water)-302.5(exchange)-301.5(to)-301.8(0%,)-300.4(switch)-302.4(off)]TJ 1.6703 -1.1116 TD [(air)-511.4(pump)-518.2(and)-511.3(UV)-516.6(ltration,)-515.9(and)-511.3(remove)-517(activated)]TJ T* [(carbon)-337.2(and)-344.3(lter)-335(materials.)-346.5(Turn)-339.6(off)-337.3(water)-342.9(pump)-339.7(be-)]TJ 0 -1.1174 TD [(fore)-356.8(emptying)-362.8(all)-356.1(water)-365.9(from)-356.9(the)-355.9(system.)-363.5(Empty)-359.4(the)]TJ 0 -1.1116 TD [(tanks)-599.9(manually.)-603.6(Remove)-604.5(ltration)-598.9(materials)-608(\(e.g.,)]TJ T* [(Siporax)-245(panels)-250.8(and)-246.4(static)-245.3(media\))-252.2(and)-246.4(autoclave)-245.2(before)]TJ 0 -1.1174 TD (disposal.)Tj -1.6703 -1.1116 TD [(\(3\))-504.3(Fill)-286.9(system)-285.2(with)-287.8(tap)-292.6(water.)-288.7(Add)-286.4(chlorine)-287.8(tablets)-292.4(\(e.g.,)]TJ 1.6703 -1.1116 TD [(Cleanline)-458.3(Chlorine)-460.5(tablets)-459.5(from)-454.8(Prime)-457.1(Sourceac-)]TJ T* [(tive)-432(ingredient)-439.9(Sodium)-434(dichloroisocyanurate\))-438.1(to)-434.3(the)]TJ 0 -1.1174 TD [(sump)-368.3(for)-371.9(a)-376.6(concentration)-376.4(of)-370.8(250300)-163.2(ppm.)-376.6(Consider)]TJ 0 -1.1116 TD [(the)-275.3(concentration)-284.3(for)-279.7(the)-275.3(total)-277.8(system)-279.4(volume)-279.8(and)-280.9(not)]TJ T* 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[(quantity)-315.6(of)-313.2(food.)-313.1(The)-316.7(biolter)-309.7(status)-318.7(can)-310.9(be)-314.4(monitored)-317.8(with)]TJ T* [(standard)-362.4(water)-365.9(quality)-360.6(tests)-363.6(to)-359.4(check)-360.3(ammonia,)-361.6(nitrite,)-364.1(and)]TJ 0 -1.1174 TD [(nitrate)-252.3(levels)-250.9(\(such)-254.1(as)-250.8(JBL/API/Sera\).)-253.2(This)-247.4(should)-254.2(be)-245.3(carried)]TJ 0 -1.1116 TD [(out)-285.7(daily)-277.6(until)-282.4(desired)-286.4(levels)-279.7(are)-282.1(reached.)]TJ 1.0137 -1.1116 TD [(Dosing)-416.4(the)-413.5(system)-411.9(weekly)-413.1(with)-414.5(nitrifying)-414.6(bacteria)-416.9(until)]TJ -1.0137 -1.1174 TD [(water)-354.4(parameters)-350.8(stabilize)-347.8(\(low)-351(level)-347.9(of)-342(nitrite,)-352.6(trace)-348.9(of)-342(ni-)]TJ 0 -1.1116 TD [(trate\))-383.5(will)-380(ensure)-377.3(sufcient)-380.7(biological)-382.5(ltration.)-377.7(If)-382.3(the)-379(bio-)]TJ T* [(logical)-551.8(ltration)-547.1(is)-543.5(working)-550.1(properly,)-549.1(the)-546(elements)-549.2(of)-543.6(a)]TJ 0 -1.1174 TD [(complete)-249.9(nitrog)22(e)15(n)-220(c)20.8(y)16.2(c)20.8(l)21.8(e)-226.9(w)21.1(i)21.8(l)16.1(l)-220.1(b)16.2(e)-221.1(i)21.8(n)-225.7(p)21.9(l)21.8(a)15(c)20.8(e)20.8(,)-222.3(a)20.8(n)16.2(d)-220(t)16.1(h)21.9(i)21.8(s)-225.5(c)20.8(a)20.8(n)-225.7(b)21.9(e)-221.1(e)15(a)20.8(s)22.1(i)16(l)21.8(y)]TJ 0 -1.1116 TD -.0216 Tc [(v)-5.4(e)-.8(r)-5.4(i).2(e)-6.6(d)-322.2(b).3(y)-322.2(t)-5.6(est)-5.5(i).2(ng)-328(for)-327.9(t).2(he)-323.4(a)-6.6(bov)-5.4(e)-323.4(t).2(h)-5.4(r).4(ee)-323.4(n)-5.4(itr)-5.4(og)-5.4(en)-322.2(co)-5.4(mpo)-5.4(und)-322.2(p)-5.4(a)-.8(-)]TJ T* -.02 Tc [(rame)-5(ters.)-340.2(I)2(f)-343.6(n)1.9(o)-337.9(t)-4(rac)-5(e)-339(of)-337.8(nitrate)-339(i)1.8(s)-337.7(d)-3.8(ete)-5(c).8(te)-5(d)-337.9(w)1.1(ith)-337.9(i)-4(ncreased)-337.9(a)-20(m)-20(-)]TJ T* 0 Tc [(monia)-301.8(and/or)-307.4(nitrite)-303.1(levels)-9(,)-297.2(then)-299.4(the)-298.3(bacte)-9.1(rial)-299.5(activi)-9.3(ty)-296.1(of)-295.9(the)]TJ 0 -1.1174 TD [(biolog)-6.9(ical)-300.7(ltrat)-8(ion)-291.4(is)-290.1(still)-299.6(not)-291.4(adequ)-7.8(ate)-293.7(\(see)-294.5()50.8(Resul)-8.7(ts)-7.9()50.8()-289.1(sec-)]TJ 0 -1.1116 TD [(ti)-8.2(on\).)]TJ 1.0137 -1.1116 TD [(Following)-506.4(the)-505.7(complete)-509.1(disinfection)-505.4(protocol,)-503.2(standard)]TJ -1.0137 -1.1174 TD [(chlorine)-287.8(tests)-283(\(Hanna)-286.2(Instruments;)-286.3(kit)-285.8(H1-3831F\))-281.3(were)-289.8(used)]TJ 0 -1.1116 TD [(to)-347.9(check)-343.1(for)-343.1(traces)-344.1(of)-342(remaining)-347.8(chlorine.)-348.9(This)-339.6(was)-345(carried)]TJ T* [(out)-297.2(daily)-289.2(for)-291.2(a)-296(week,)-293.3(and)-292.4(samples)-293.2(were)-295.6(collected)-297.2(from)-293.5(mul-)]TJ 0 -1.1174 TD [(tiple)-387.2(locations)-386.8(on)-382.3(the)-384.7(system)-383.1(\(e.g.,)-389.1(sump,)-383.3(UV)-384.1(outow)-381.8(tap,)]TJ 0 -1.1116 TD [(different)-271.6(tanks,)-263.5(and)-263.6(individual)-266.1(tank)-264.9(taps\).)-263.4(Test)-265.9(kit)-262.7(sensitivity)]TJ T* [(was)-396.8(also)-391.4(tested)-389.1(by)-393.9(comparing)-393.7(tap)-390.5(water)-388.9(and)-390.4(ltered)-396(water)]TJ 0 -1.1174 TD [(when)-403.9(testing)-395(our)-400.7(system)-400.4(water.)-398.1(As)-400.3(different)-398.3(systems)-401.3(were)]TJ 0 -1.1116 TD [(used)-391.3(for)-383.4(sh)-383.1(of)-382.3(different)-392.5(ages,)-384.3(sh)-383.1(were)-387.7(introduced)-388(to)-388.2(the)]TJ T* [(restarted)-243.8(systems)-234.3(in)-238.5(differing)-235.9(ways.)-240.1(Z-hab)-232.5(recirculating)-244.1(units)]TJ T* [(were)-203.4(used)-201.2(for)-199.1(the)-200.4(rearing)-200.2(of)-198(larvae)-206(sh)-198.8(from)-201.4(a)-198.1(new)-201.2(population)]TJ 0 -1.1174 TD [(from)-282(a)-284.5(new)-281.8(colony.)]TJ 1.0137 -1.1116 TD [(For)-538.8(the)-540.2(introduction)-539(of)-532.1(adult)-542.6(sh)-532.8(\(predi)-7.8(sinfection,)-535.3(in-)]TJ -1.0137 -1.1116 TD [(house,)-436.1(adult)-433.2(wild)-431.8(population\),)-431.8(300)-170.3(L)-428.5(glass)-432.6(aquarium)-438.7(units)]TJ 0 -1.1174 TD [(were)-186.1(used.)-181.6(In)-180.7(two)-182.8(of)-180.7(these)-180.6(tanks,)-182.8(neither)-183.1(cautious)-184(introduction)]TJ 0 -1.1116 TD [(nor)-481.3(decreased)-487.8(feeding)-476.7(steps)-484.5(were)-479.9(implemented.)-482.7(Approxi-)]TJ T* [(mately)-511.5(500600)-503.1(sh)-504(were)-502.9(simultaneously)-508.7(introduced)-508.9(per)]TJ 0 -1.1174 TD [(tank,)-349(and)-344.3(nitrifying)-351.3(bacteria)-347.8(displayed)-346.4(signs)-345.1(of)-347.7(inefciency)]TJ 0 -1.1116 TD [(\(see)-380.9()50.8(Results)45.7()-381.2(section\).)-377.5(To)-377.8(prevent)-378.8(biological)-382.5(lter)-381.1(issues)]TJ T* [(from)-264.8(arising)-268.1(when)-265.6(introducing)-267.1(a)-261.4(high)-263.7(number)-268.2(of)-261.4(sh)-262.1(at)-268.4(once)]TJ T* [(for)-354.6(the)-350.2(remaining)-359.3(experimental)-353.5(group,)-354.5(these)-353.4(sh)-348.5(were)-358.9(split)]TJ 0 -1.1174 TD [(into)-263.8(four)-257.8(300)-164.6(L)-261.5(glass)-259.8(aquarium)-260.1(units,)-256.7(and)-257.9(a)-261.4(new)-258.8(introduction)]TJ 0 -1.1116 TD [(protocol)-188.8(was)-189.5(followed:)-190.9(A)-186.3(maximum)-191.2(of)-186.5(100)-187.6(sh)-181.5(were)-191.9(added)-186.4(to)]TJ T* [(each)-313.2(tank)-311(every)-313.1(other)-312(day)-309.7(while)-313.1(implementing)-312.3(a)-313.3(half-ration)]TJ 0 -1.1174 TD [(feeding)-286.6(regime)-281(for)-279.7(the)-281.1(rst)-286.4(week.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 313.0582 237.9968 Tm [(Postdisinfection)-340.6(diagnostics)]TJ /F5 1 Tf 9.843 0 0 9.843 323.0361 221.5559 Tm [(The)-276.4(QM)-268.9(Diagnostics)-280.3(Circulum)-271.7(Kit)-275.1(included)-276.3(test)-270.6(tubes)-271.5(for)]TJ -1.0137 -1.1174 TD [(the)-217.7(following:)-214(one)-211.8(biolm)-215.3(swab,)-211.3(one)-217.6(tube)-213(for)-210.6(a)-215.4(water)-216.1(sample,)]TJ 0 -1.1116 TD [(one)-194.5(sh)-187.3(sample,)-192.1(and)-188.8(one)-194.5(tube)-190(for)-187.5(a)-192.3(food)-188.7(sample.)-192.1(In)-192.2(November)]TJ T* [(of)-336.2(2017,)-330.4(6)-335.2(months)-330.3(after)-336.2(the)-332.9(sterilization)-335.1(of)-336.2(the)-332.9(facility,)-337.6(one)]TJ 0 -1.1174 TD [(full)-367.5(testing)-360.4(kit)-360.7(was)-368(lled)-358.1(\(separately)-368.1(for)-360.3(each)-365(of)-365(the)-361.7(stand-)]TJ 0 -1.1116 TD [(alone)-347.8(and)-344.3(recirculation)-347.7(unit\),)-343.2(as)-343(instructed)-347.6(by)-342(the)-338.7(company)]TJ T* [(and)-304(returned)-299.2(for)-302.7(full)-298.4(diagnostic)-301.5(investigation.)-302.8(Food)-298(samples)]TJ 0 -1.1174 TD [(included)-362.7(both)-361.6(live)-362.9(and)-355.8(dry)-360.4(food)-361.5(sample)-361.4(types.)-361.4(One)-362.4(test)-357(kit)]TJ 0 -1.1116 TD [(conta)-8(ined)-230.3(freshly)-233.4(hatched)-236(artemia)-231.5(nauplii)-233.9(and)-234.9(in)-232.7(the)-229.2(other)-237.1(kit)]TJ T* [(dry)-285.5(food)-280.8(\(ZM400\))-280.5(for)-279.7(testing.)]TJ 1.0137 -1.1174 TD [(The)-449.2(biolm)-445.7(swabs)-444.9(were)-445.3(collected)-452.7(from)-443.3(the)-442.3(part)-449.2(of)-445.7(the)]TJ -1.0137 -1.1116 TD [(recirculati)-11.5(on)-249.9(unit)-258.1(that)-259.3(displays)-257.5(the)-252.3(largest)-257.7(buildup)-255.6(of)-255.6(organic)]TJ T* [(matter,)-370.9(the)-361.7(spillway)-368.1(container)-365(\(Fig.)-364.9(2\).)-362.7(Two)-368.2(sh)-360(were)-370.4(ran-)]TJ T* [(domly)-410(selected)-410.9(from)-403(the)-407.8(system.)-403.8(The)-408.8(sh)-406.1(were)-405(culled)-412.4(ac-)]TJ 0 -1.1174 TD [(cording)-694.4(to)-687.7(United)-688.5(Kingdom)-689.6(Home)-687.3(Ofce)-688.2(Schedule)-691.9(1)]TJ 0 -1.1116 TD [(guidelines)-497.4(for)-492.8(humane)-499.7(euthanasia)-493.8(\(overdose)-495.8(of)-497.5(350)-164.6(mg/L)]TJ /F4 1 Tf 8.9663 0 0 8.9663 62.022 749.6502 Tm (82)Tj 40.846 0 TD (RA)Tj .9168 .2087 TD ()Tj .5185 -.2087 TD [(CZ,)-334.5(DWYER,)-341.5(AND)-330.9(KILLEN)]TJ ET endstream endobj 70 0 obj <>stream /GS1 gs BT /F5 1 Tf 9.843 0 0 9.843 59.7543 725.1588 Tm 0 0 0 rg 0 Tc 0 Tw [(buffered)-229.9(Tricaine)-232.6(solution)-227.9(\()-81.7(MS-222\))-227.3(for)-227.9(30)-169.2(min,)-221.2(followed)-229.9(by)]TJ 0 -1.1174 TD [(conrmatio)-8.9(n)-196.9(o)0(f)-198(death\).)-196.8(The)-201.5(sh)-198.8(were)-197.6(stored)-203.5(and)-194.5(processed)-203.1(by)]TJ 0 -1.1116 TD [(QM)-297.7(Diagnostics)-297.5(as)-291.2(per)-298.2(company)-293.5(diagnostic)-295.8(guidelines.)-299.2(Cir-)]TJ T* [(culum)-399.7(sampling)-397.2(prole)-399.4(consists)-395.5(of)-399.6(high)-396.2(sensitivity,)-397.1(highly)]TJ T* [(specic,)-250.6(and)-246.4(fast)-247.4(TaqMan)-252.9(qPCR)-248.2(and)-246.4(chemical)-251.1(assays,)-247.9(which)]TJ 0 -1.1174 TD [(are)-524(all)-523.1(validated.)-518.3(In)-520.5(the)-523(sample)-522.7(preparation)-522.7(process,)-519.9(the)]TJ 0 -1.1116 TD [(company)-299.3(grinds)-294.5(and)-292.4(homogenizes)-299(the)-298.3(whole)-295.7(body)-292.4(of)-295.9(sh)-290.9(in)]TJ T* [(buffer)-284.1(and)-280.9(uses)-281.6(this)-280.9(for)-279.7(total)-283.5(nucleic)-282.2(acid)-283.3(purication.)]TJ 1.0137 -1.1174 TD [(Following)-396.9(positive)]TJ /F6 1 Tf 8.0809 0 TD (Mycobacterium)Tj /F5 1 Tf 6.687 0 TD [(spp.)-392.4(detection)-399.7(in)-388.2(the)]TJ -15.7816 -1.1116 TD [(standard)-252.9(kit)-245.5(prole,)-247.3(a)-244.2(decision)-247.4(was)-247.1(made)-249.9(to)-244.2(perform)-246.1(species-)]TJ T* [(specic)-316.2(testing)-314.3(for)]TJ /F6 1 Tf 7.839 0 TD (Mycobacterium)Tj /F5 1 Tf 6.6064 0 TD [(spp.)-311.8(screening,)-310.5(to)-313.4(detect)]TJ -14.4453 -1.1174 TD [(the)-373.2(possible)-372.7(presence)-370.3(of)-370.8(the)-373.2(six)-366.1(most)-368.4(common)-373.1(species:)]TJ /F6 1 Tf 23.2116 0 TD (M.)Tj -23.2116 -1.1116 TD (chelonae)Tj /F5 1 Tf 3.6286 0 TD (,)Tj /F6 1 Tf .6105 0 TD [(M.)-368.5(fortuitum)]TJ /F5 1 Tf 5.178 0 TD (,)Tj /F6 1 Tf .6163 0 TD [(M.)-362.7(haemophilum)]TJ /F5 1 Tf 6.8944 0 TD (,)Tj /F6 1 Tf .6163 0 TD [(M.)-362.7(marinum)]TJ /F5 1 Tf 5.0513 0 TD (,)Tj /F6 1 Tf .6163 0 TD (M.)Tj -23.2116 -1.1116 TD (peregrinum)Tj /F5 1 Tf 4.6711 0 TD [(,)-279.9(and)]TJ /F6 1 Tf 2.2578 0 TD [(M.)-282.1(abscessus)]TJ /F5 1 Tf 5.2989 0 TD (.)Tj -11.2141 -1.1116 TD [(In)-370.8(June)-368.2(of)-365(2018,)-370.7(a)-365.1(second)-371.6(round)-368.3(of)-365(diagnostic)-370.6(tests)-369.4(was)]TJ -1.0137 -1.1174 TD [(carried)-235.9(out)-222.3(on)-226.8(both)-229.2(systems.)-232(This)-224.4(was)]TJ /F11 1 Tf 15.1941 0 TD (*)Tj /F5 1 Tf 1.0022 0 TD [(1)-225.7(year)-231.3(after)-226.8(carrying)]TJ -16.1963 -1.1116 TD [(out)-452.7(the)-453.9(disinfection)-459.3(protocol)-453.7(and)-453.7(6)-450.4(months)-457(from)-449.1(the)-453.8(rst)]TJ T* [(postdisinfection)-446.4(diagnostic)-445.5(test.)-435.3(Following)-443(the)-442.3(recommen-)]TJ 0 -1.1174 TD [(dations)-193.2(of)-192.2(two)-188.6(detailed)-197(methodology)-193.3(articles)-191(providing)-194.5(newly)]TJ 0 -1.1116 TD [(established)-309.4(health)-308.7(monitoring)-306.4(techniques)-302.6(in)-307.6(zebrash)-305.7(facili-)]TJ T* [(ties,)-360.4(we)-355.6(used)-351(these)-359.1(environmental)-359.2(screening)-358.9(techniques)-354.4(for)]TJ 0 -1.1174 TD [(our)-285.5(samples.)]TJ 7.3823 0 0 6.5614 109.9275 499.4077 Tm (24,25)Tj 9.843 0 0 9.843 69.7323 484.0439 Tm -.0217 Tc 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Tc [(s)271.3(e)275.7(c)275.7(t)271(i)276.7(o)271.1(no)276.8(ft)271(h)276.8(e)-.8(s)271.3(u)276.8(m)275.6(p)276.8(\))271.1(.)-2(T)275.7(h)276.8(e)271.2(f)276.9(t)276.7(h)-5.4(s)277(w)276(a)269.9(bw)276(a)269.9(s).6(t)276.7(a)275.7(k)271.1(e)275.7(nf)271.1(r)276.9(o)276.8(mt)271(h)276.8(e)-.8(i)271(n)276.8(n)276.8(e)269.9(r)276.9(-)]TJ T* -.0216 Tc [(e)-6.6(n).3(d)-287.6(s)-5.2(ect)-5.5(ion)-293.4(o).3(f)-287.6(t)-5.5(he)-288.8(b)-5.4(ack)-5.4(o)-5.4(w)-288.5(p).3(ip)-5.4(e)-288.8(f).4(r)-5.4(o).3(m)-288.9(o)-5.4(ne)-288.8(Z)-6.6(-).4(ha)-6.6(b)-287.6(u).3(n)-5.4(i).2(t)-287.8(\()-5.4(Fig)-5.4(.)-290(2\),)]TJ 0 -1.1174 TD [(t)-5.4(h).4(e)-231.1(u)-5.3(ni)-5.4(t)-230.1(w)-.4(h)-5.3(i).3(ch)-235.7(hou)-5.3(se)-6.5(d)-229.9(t).3(h)-5.3(e)-231.1(maj)-5.4(o).4(r)-5.3(ity)-235.7(of)-235.7(the)-236.9(r).5(ea)-6.5(red)-235.7().6(s)-5.1(h).4(.)-232.3(E)-.7(a)-6.5(c)-.7(h)-229.9(t)-5.4(ime)]TJ 0 -1.1116 TD -.0201 Tc [(the)-183.6(b)1.8(iolm)-177.9(s)-3.7(wab)-182.5(w)1(a)-5.1(s)-182.3(taken,)-184.8(the)-183.6(c).7(ollection)-182.5(a).7(rea)-183.6(w)1(as)-182.3(c)-5.1(onc)-5.1(entrated)]TJ T* -.0216 Tc [(a)-6.6(t)-282(the)-283(a)-6.6(ir)-5.4(w)-.5(at)-5.5(er)-281.8(in)-5.4(te)-6.6(rfa)-6.6(ce)-283(of)-281.9(t)-5.5(h).3(e)-283(s).5(u)-5.4(m)-.9(p)-281.9(t).2(a)-6.6(nk.)]TJ 7.3823 0 0 6.5614 224.1637 400.7621 Tm -.0239 Tc (25)Tj 9.843 0 0 9.843 69.7323 385.3983 Tm 0 Tc [(Fi)20.9(ve)-291.3(re)19.7(ar)19.7(in)20.7(g)-294.8(t)0(a)13.8(n)0(k)20.8(s)-294.6(fr)20.9(om)-291.4(th)20.7(e)-296(u)0(n)20.8(i)0(t)20.6(s)-300.4(ho)20.8(us)21(in)20.7(g)-300.6(t)0(h)20.7(e)-296(ne)19.7(w)-295.7(p)0(o)15.1(p)0(u)20.8(l)0(a)19.6(-)]TJ -1.0137 -1.1116 TD [(ti)14.8(on)-376.6(we)18.8(re)-377.7(se)14.1(le)19.6(ct)19.5(ed)-377.7(ra)19.7(nd)20.8(om)19.6(ly)-382.5(fo)20.9(r)-387()0(s)15.4(h)-387(sl)20.9(ud)20.8(ge)-377.7(co)13.9(ll)20.6(ec)18.5(ti)20.6(on)20.8(.)-389.3(T)0(h)19.6(e)]TJ T* [(sm)19.8(al)13.8(le)19.6(r)-254.5(s)0(i)20.9(z)0(e)18.5(d)-260.3(ba)19.7(f)21(e)-255.7(m)0(e)18.4(s)0(h)-243.9(\()0(4)15.1(0)0(0)20.8()0(7)20.8(0)0(0)]TJ /F12 1 Tf 14.1919 0 TD (l)Tj /F5 1 Tf .5414 0 TD [(m\))-245.3(us)15.3(ed)-245.3(in)-244.2(re)19.7(ar)19.7(in)15(g)-254.5(t)0(a)19.6(n)0(k)20.8(s)]TJ -14.7333 -1.1174 TD [(ac)18.5(cu)13.9(mu)19.6(la)19.5(te)19.5(s)-311.9(s)0(l)20.9(u)0(d)20.8(g)0(e)-302.9(m)0(o)19.6(r)0(e)-302.8(r)0(e)19.7(a)0(d)19.7(i)0(l)14.8(y)0(,)-298.3(r)0(e)19.7(s)0(u)21(l)0(t)14.8(i)0(n)20.7(g)-312.1(in)-296.1(a)-313.3(c)0(o)19.7(n)0(c)19.7(e)0(n)13.9(t)0(r)20.7(a)0(t)19.5(e)0(d)]TJ 0 -1.1116 TD [(la)13.8(ye)19.7(r)-191.1(a)0(t)-187.8(t)0(h)20.7(e)-192.3(bo)20.8(tt)14.8(om)-182(of)-186.5(ea)18.5(ch)-181.9(ta)19.6(nk)15.1(.)-193.5(T)0(h)19.6(i)0(s)-180.7(m)0(a)18.4(d)0(e)-187.7(f)0(o)20.9(r)-196.9(st)20.9(ra)19.7(ig)20.7(ht)20.7(fo)15.1(rw)20(ar)19.7(d)]TJ T* [(co)13.9(ll)20.6(ec)18.5(ti)20.6(on)20.8(,)-458.4(a)0(n)19.7(d)-456.1(sa)14.1(m)15(p)0(l)14.9(e)0(s)-446.7(w)0(e)18.8(r)0(e)-446.8(s)0(t)15.1(o)0(r)20.9(e)0(d)-446.9(i)0(n)-451.6(1)0(5)-146.2(m)0(L)-448.1(t)0(u)20.7(b)0(e)19.7(s)0(.)-447.8(E)0(a)18.5(c)0(h)]TJ T* [(sl)15.1(ud)20.8(ge)-418.1(sa)19.9(mp)19.6(le)-418.2(sw)20.2(ab)-418.1(wa)18.8(s)-427.1(t)0(h)20.7(e)0(n)-418.1(p)0(r)20.9(e)0(p)19.7(a)0(r)19.7(e)0(d)-418.1(f)0(o)15.1(r)-421.5(PC)14.2(R)-422.6(f)0(o)20.9(l)0(l)14.8(o)0(w)20(i)0(n)20.7(g)]TJ 0 -1.1174 TD [(ro)15.1(ut)20.7(in)20.7(e)-307.5(p)0(r)20.9(o)0(t)20.7(o)0(c)13.9(o)0(l)20.7(s)0(.)]TJ 7.3823 0 0 6.5614 128.4094 313.0582 Tm -.0173 Tc [(24)-17.3(,25)]TJ 9.843 0 0 9.843 147.685 308.6928 Tm 0 Tc [(Al)19.8(l)-306.5(1)0(0)-295.9(s)0(a)19.9(m)0(p)19.6(l)0(e)19.6(s)-311.9(\()21(ve)-297.1(bi)20.7(o)21(lm)-303.1(sw)20.2(ab)-297.1(an)19.7(d)]TJ -8.9333 -1.1116 TD [(v)15.2(e)-267.2(s)0(l)15.1(u)0(d)20.8(g)0(e)-256.8(s)0(w)14.4(a)0(b)19.7(\))-271.8(we)18.8(re)-262.5(se)19.9(nt)-261.5(to)-255.8(QM)-263.2(Di)19.8(ag)19.7(no)15.1(st)20.9(ic)19.6(s)-271.6(f)0(o)20.9(r)-271.8(PC)20(R)-272.8(t)0(e)19.6(s)0(t)20.9(-)]TJ T* [(in)15(g.)-275.2(Sa)19.8(mp)19.6(le)19.5(s)-283.1(w)0(e)13(r)0(e)-274(s)0(e)19.9(n)0(t)-278.8(t)0(o)-273(t)0(h)15(e)-284.5(co)19.7(mp)19.6(an)19.7(y)-289.1(i)0(n)-273(s)0(e)19.9(p)0(a)19.7(r)0(a)14(t)0(e)-274.2(t)0(u)20.7(b)0(e)19.7(s)-288.9(an)19.7(d)]TJ 0 -1.1174 TD [(th)15(en)-400.8(po)20.8(ol)14.9(ed)-400.8(by)-405.4(th)20.7(e)-411.2(c)0(o)19.7(m)0(p)19.6(a)0(n)13.9(y)-410(up)20.8(on)-405.4(re)19.7(ce)18.5(ip)20.7(t.)-407.8(Po)21(ol)20.7(ed)-406.5(sa)19.9(mp)19.6(le)19.5(s)]TJ 0 -1.1116 TD [(we)18.8(re)-256.8(ba)19.7(se)19.9(d)-260.3(o)0(n)-255.6(a)0(n)-251(i)0(n)20.7(-)0(h)15.1(o)0(u)20.8(s)0(e)-250.8(v)0(a)19.7(l)0(i)20.6(d)0(a)13.9(t)0(e)19.6(d)-260.3(po)20.8(ol)20.7(in)15(g)-260.3(p)0(r)20.9(o)0(t)20.7(o)0(c)19.7(o)0(l)-255.8(b)0(y)-249.9(Q)0(M)]TJ T* [(Di)14.1(ag)19.7(no)20.8(st)20.9(ic)19.6(s)-358(w)0(h)20(e)0(r)19.7(e)-359.4(v)21(e)-359.4(i)0(n)20.7(d)0(i)20.7(v)0(i)20.7(d)0(u)20.8(a)0(l)-349.1(s)0(a)19.9(m)0(p)19.6(l)0(e)19.6(s)-358(we)18.8(re)-348.9(po)20.8(ol)20.7(ed)-348.9(in)20.7(to)]TJ 0 -1.1174 TD [(on)15.1(e)-267.2(t)0(e)19.5(s)0(t)-261.3(s)0(a)14.1(m)15(pl)14.9(e)-267.2(f)0(o)15.1(r)-266(ea)18.5(ch)-262.5(ca)18.5(te)19.6(go)15.1(ry)-255.6(\(b)15.1(io)20.7(l)20.9(m)-273(o)0(r)-255.6(s)0(l)15.1(u)0(d)20.8(g)0(e)19.7(\))0(.)]TJ 1.0137 -1.1116 TD [(For)-400.5(PCR,)-406.1(all)-402.1(samples)-402.7(were)-405(pooled)-399.6(in)-399.7(sterile)-407.6(phosphate-)]TJ -1.0137 -1.1116 TD [(buffered)-322.1(saline)-320(buffer,)-322.1(and)-315.5(total)-318.1(nucle)-8(ic)-314.5(acid)-323.6(was)-316.2(extracted.)]TJ 0 -1.1174 TD [(Swabs)-398.8(were)-393.5(suspended)-397.8(and)-396.1(mixed)-393.9(and)-396.1(then)-391.6(175)]TJ /F12 1 Tf 20.2799 0 TD (l)Tj /F5 1 Tf .5472 0 TD [(L)-393.9(o)0(f)-393.8(the)]TJ -20.8271 -1.1116 TD [(pooled)-295.9(sample)-292.3(diluent)-291.5(was)-293.1(used)-293.4(for)-291.2(the)-286.8(DNA)-293.9(extraction.)-294.8(As)]TJ T* [(mentioned)-301.7(previously,)-303.6(the)-304.1(PCR)-299(prole)-301.5(consists)-303.4(of)-295.9(high)-298.3(sen-)]TJ T* [(sitivity,)-208.5(highly)-196.9(specic,)-210.2(and)-200.3(fast)-201.3(TaqMan)-206.9(qPCR)-202.1(and)-206.1(chemical)]TJ 0 -1.1174 TD [(assays,)-242.2(which)-238.1(are)-236(all)-235.1(validated)-238.4(by)-238.3(QM)-234.4(Diagnostics)-239.9(Company)]TJ 0 -1.1116 TD (standards.)Tj /F4 1 Tf 8.9663 0 0 8.9663 59.7543 133.285 Tm (Results)Tj /F2 1 Tf 0 -1.8336 TD (Histology)Tj /F5 1 Tf 9.843 0 0 9.843 69.7323 100.4031 Tm [(Infected)-334.9(individuals)-330.4(in)-330.6(the)-332.9(original)-332.9(wild)-328.1(parental)-335.1(popula-)]TJ -1.0137 -1.1116 TD [(tion)-269.6(showed)-273.4(extensive)-272.7(granulomatous)-271.3(inammation)-270.6(forming)]TJ 0 -1.1174 TD [(sheets)-244.8(of)-244.1(macrophages)-248.2(within)-244(the)-246.5(dermis,)-242.7(musculature,)-244.9(liver,)]TJ 0 -1.1116 TD [(and)-332.8(peritoneum)-331.6(and)-332.8(extending)-329.3(to)-330.6(all)-333(visceral)-334.9(organs.)-333.5(In)-324.7(the)]TJ T* [(majority)-195.9(of)-186.5(sh,)-190.7(distinct)-188.9(granuloma)-192.1(formation)-191.1(was)-189.5(also)-189.8(noted.)]TJ 25.5097 67.9242 TD [(Macrophages)-282.4(generally)-284.3(had)-275.2(a)-278.7(nely)-283.1(granular)-276.1(eosinophilic)-286.6(to)]TJ 0 -1.1174 TD [(basophilic)-186.3(cytoplasm,)-184(suggestive)-186(of)-180.7(bacterial)-187.7(infection.)-183.1(Large)]TJ 0 -1.1116 TD [(numbers)-574.3(of)-572.4(intralesional)-576.9(acid-fast,)-575.5(predominantly)-576.9(Gram-)]TJ T* [(positive)-200.2(bacteria)-209.5(were)-203.4(seen)-202.4(within)-203.7(sheets)-204.5(of)-203.8(macrophages)-207.9(and)]TJ T* [(within)-278.6(distinct)-286.8(granulomas.)]TJ 1.0137 -1.1174 TD [(Histological)-379.7(examination)-385.8(of)-382.3(F1sh)-379.4(revealed)-382.2(copious)-383.2(ba-)]TJ -1.0137 -1.1116 TD [(sophilic,)-451.3(granular)-454.7(bacterial)-458.4(colonies)-454.7(within)-451.4(the)-453.8(epidermis,)]TJ T* [(muscle)-401.8(tissues,)-409.4(liver,)-402(and)-401.9(peritoneum)-406.5(and)-401.9(extending)-404.2(to)-405.5(all)]TJ 0 -1.1174 TD [(visceral)-438.6(organs.)-437.2(Often)-433.9(associated)-441.8(with)-431.8(the)-436.6(granuloma)-439.8(for-)]TJ 0 -1.1116 TD [(mations)-476.7(were)-485.6(extensive)-480(inammations.)-482.3(In)-474.5(ve)-482.4(of)-474.5(the)-482.6(six)]TJ T* [(samples,)-319.7(ZN)-315.3(stains)-318.7(were)-318.6(strongly)-314.1(positive)-315.4(for)-320(mycobacteria)]TJ 0 -1.1174 TD [(within)-359.2(the)-361.7(lesions.)-363.5(A)-364.8(lack)-364(of)-359.3(staining)-361.5(and)-361.6(bacteria)-370.8(identi-)]TJ 0 -1.1116 TD [(cation)-279.9(within)-278.6(some)-277.3(lesions)-279.4(occurred,)-280.6(but)-274.1(this)-275.2(was)-281.6(thought)-278.7(to)]TJ T* [(be)-274.1(artifact)-271.8(rather)-273.9(than)-276.4(a)-273(secondary)-272.4(bacterial)-279.9(infection.)-275.2(It)-273(was)]TJ T* [(advised)-344(by)-342(histology)-338.4(staff)-346.4(that)-339.9(a)-342.1(severe)-343.9(systemic)-346.3(mycobac-)]TJ 0 -1.1174 TD [(terial)-354.9(infection)-352.4(can)-351.2(often)-358.1(be)-348.9(associated)-355.4(with)-356.9(epidemics)-354.5(and)]TJ 0 -1.1116 TD [(high)-396.2(mortalities)-402(if)-394(the)-402(disease)-402.6(pressure)-396.6(is)-399.6(sufcient)-398(and/or)]TJ T* [(there)-474.5(are)-477.9(underlying)-478.9(stressors.)-474.6(It)-474.6(was)-477.4(also)-477.8(noted)-473.3(that)-478.1(the)]TJ 0 -1.1174 TD [(weight)-400.6(loss)-395.8(and)-396.1(mortalities)-402(seen)-398.2(in)-399.7(the)-396.3(source)-400.4(populations)]TJ 0 -1.1116 TD [(were)-284(likely)-278.9(related)-287.9(to)-278.8(this.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 310.8472 493.1715 Tm (Bacteriology)Tj /F5 1 Tf 9.843 0 0 9.843 320.8251 476.7306 Tm -.0215 Tc [(Bact)-5.4(er)-5.3(iol)-5.4(ogy)-304.8(res)-5.1(u).4(lt)-5.4(ed)-299.1(i)-5.4(n)-299.1(mi)-5.4(xed)-299.1(g)-5.3(ro)-5.3(wth)-299.1(o)-5.3(n)-299.1(a)-.7(l)-5.4(l)-299.2(sa)-6.5(mpl)-5.5(e)-.7(s.)-301.4(S)-5.2(ub-)]TJ -1.0137 -1.1174 TD [(cul)-5.5(t).2(u)-5.4(r).4(e)-260(o).3(f)-258.8(t)-5.5(he)-260(do)-5.4(mina)-6.6(nt)-259(co)-5.4(lon)-5.4(y)-258.8(typ)-5.4(e)-260(fr)-5.4(om)-260.1(all)-259(t)-5.5(hre)-6.6(e)-260(sh)-258.8(s)-5.2(a)-.8(mp)-5.4(les)]TJ 0 -1.1116 TD [(was)-304.7(i).2(d)-5.4(e)-.8(nt)-5.5(ie)-6.6(d)-299.2(as)-304.7(a)-300.3(G)-.5(r)-5.4(a)-.8(m)-300.4(n).3(e)-6.6(g).3(at)-5.6(ive)-6.6(,)-301.5(sho)-5.4(r).4(t)-299.3(r)-5.4(od)-5.4(,)-295.7(o)-5.4(xi)-5.5(das)-5.2(e)-300.3(po)-5.4(sit)-5.5(i).2(v)-5.4(e)-.8(,)]TJ T* -.0195 Tc [(fermentative)-229.1(motile)-229.1(bacterial)-228.1(s)]TJ 11.6404 0 TD -.0202 Tc [(pe)-5.2(cie)-5.2(s)-228.5(with)-228.6(a)-229.8(b)-4(ioc)-5.2(h)1.7(emical)-234.5(prole)]TJ -11.6404 -1.1116 TD -.0216 Tc [(mat)-5.5(c)-.8(hi)-5.6(ng)-195.5(m)-6.6(o).3(ti)-5.6(le)]TJ /F6 1 Tf 6.4221 0 TD -.0201 Tc [(Ae)-5.1(romonas)]TJ /F5 1 Tf 4.5674 0 TD -.0167 Tc [(sp)5.2(eci)5.1(e)4.1(s,)-192.9(l)5.1(i)-.7(k)5.2(e)4.1(ly)]TJ /F6 1 Tf 5.5524 0 TD -.0216 Tc [(A.)-197.8(hy)-6.6(dro)-5.4(p).3(h)-5.4(ila)]TJ /F5 1 Tf 5.2183 0 TD .1705 Tc [(.N)185.8(o)192.4(n)186.7(e)]TJ -21.7602 -1.1174 TD -.0216 Tc [(of)-339.5(th)-5.4(e)-334.9(s)-5.2(amp)-5.4(l).2(es)-339.3(wa)-6.6(s)-339.3(pos)-5.2(it)-5.5(ive)-340.6(f).4(o)-5.4(r)-333.7(r)-5.4(e)-.8(c)-6.6(ove)-6.6(ry)-339.5(and)-339.5(i).2(d)-5.4(e)-.8(nt)-5.6(i)-5.3(cat)-5.5(ion)-339.5(o).3(f)]TJ /F6 1 Tf 0 -1.1116 TD -.0214 Tc [(Myc)-6.4(oba)-5.2(cte)-6.4(r).7(i)-5.4(u).5(m)]TJ /F5 1 Tf 6.3126 0 TD -.0218 Tc [(sp)-5.6(p.)-284.4(usi)-5.7(n).1(g)-282.1(c)-1(o)-5.6(nve)-6.8(nti)-5.7(o).1(n)-5.6(a)-1(l)-282.2(t)0(es)-5.4(ts)-5.4(.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 310.8472 387.2125 Tm 0 Tc [(System)-335.3(disinfection)-335.3(protocol)]TJ /F5 1 Tf 9.843 0 0 9.843 320.8251 370.7716 Tm [(From)-241.6(chlorine)-241.8(testing,)-242.9(no)-244.1(trace)-245.2(of)-238.3(chlorine)-247.5(was)-241.3(detected)-249.9(at)]TJ -1.0137 -1.1174 TD [(any)-350(point,)-354.8(even)-358.1(during)-352.3(initial)-355.1(sampling.)-354.5(Test)-352.3(kit)-354.9(sensitivity)]TJ 0 -1.1116 TD [(results)-584.7(showed)-584.4(varying)-579.2(amounts)-586(of)-583.9(detectable)-587.4(traces)-586(of)]TJ T* [(chlorine)-218.7(between)-219.6(water)-221.9(sources.)-220.4(As)-216(previously)-219.6(described,)-224.1(the)]TJ 0 -1.1174 TD [(introduction)-314.4(of)-319(adult)-318(sh)-314(into)-315.7(the)-315.6(rst)-315.2(two)-315.3(tanks)-317.6(did)-320.2(not)-314.5(in-)]TJ 0 -1.1116 TD [(volve)-289(either)-285.6(cautious)-293.4(introduction)-291.3(or)-284.4(decreased)-292(initial.)-289.4(After)]TJ T* [(this)-545.9(introduction,)-548.2(ammonia,)-551.7(nitrite,)-548.4(and)-545.9(nitrate)-551.8(remained)]TJ T* [(minimal)-224.8(for)-227.9(several)-230(days,)-227.7(then)-224.6(ammonia)-231.5(peaked)-229(for)-222.1(23)-227.9(days)]TJ 0 -1.1174 TD [(\(0.40.6)-169(mg/L\),)-316.7(but)-320.2(after)-318.9(50%)-315.4(water)-319.8(change)-321.1(returned)-322.2(to)-319.1(un-)]TJ 0 -1.1116 TD [(detectable)-224.6(levels.)-219.8(This)-218.6(was)-218.3(quickly)-216.5(followed)-224.2(by)-215.3(a)-221.1(nitrite)-216.7(peak)]TJ T* [(\(0.81)-164.4(mg/L\))-284.5(with)-282(undetectable)-284.4(nitrate)-281.1(levels.)]TJ 1.0137 -1.1174 TD [(This)-449(demonstrated)-450(that)-455.1(the)-448.1(nitrifying)-449.2(bacteria)-457.2(were)-451.1(be-)]TJ -1.0137 -1.1116 TD [(ginning)-353.6(to)-353.7(colonize)-358.1(the)-355.9(system,)-357.8(but)-354.8(the)-350.2(number)-360.3(of)-353.5(bacteria)]TJ T* [(was)-408.3(not)-406.6(yet)-407.8(sufcient)-409.5(enough)-411(to)-405.5(manage)-414.5(the)-407.8(high)-407.7(load)-408.9(of)]TJ 0 -1.1174 TD [(nitrogen)-206(waste)-204.5(being)-208.4(produced)-201.2(by)-203.8(the)-206.2(sh.)-202.2(After)-209.2(another)-206(50%)]TJ 0 -1.1116 TD [(of)-468.7(water)-475.3(change)-476.6(and)-471(the)-471.1(addition)-471.1(of)-474.5(additional)-474.6(nitrifying)]TJ T* [(bacteria,)-264.8(nitrite)-262.8(levels)-262.4(started)-263.5(to)-261.5(decrease)-267.8(and)-257.9(in)-261.5(a)-261.4(weak)-261(time)]TJ 0 -1.1174 TD [(stabilized)-343.1(below)-347.5(0.1)-166.9(mg/L;)-342.3(nitrate)-344.5(levels)-343.1(reached)-346.4(and)-344.3(stabi-)]TJ 0 -1.1116 TD [(lized)-261.5(at)-262.7(15)-164.6(mg/L.)-263.8(After)-261(using)-258.9(the)-263.8(new)-264.5(introduction)-262.5(protocol)]TJ T* [(of)-353.5(splitting)-355.9(the)-350.2(experimental)-359.3(sh)-354.3(into)-350.2(four)-355.7(300)-164.6(L)-353.6(tanks)-358(and)]TJ T* [(introducing)-261.3(a)-255.7(maximum)-260.4(of)-255.6(100)-262.5(individuals)-255.5(per)-263.6(every)-255.5(second)]TJ 0 -1.1174 TD [(day)-396.1(with)-403(a)-393.9(half-ration)-406.4(feeding)-401.8(regime,)-399.6(there)-399.6(were)-399.2(no)-399.6(high)]TJ 0 -1.1116 TD [(peaks)-353.2(of)-353.5(ammonia)-352.5(detected,)-357(and)-350(the)-355.9(detectable)-357(level)-353.7(of)-353.5(ni-)]TJ T* [(trogen)-272.8(compounds)-271.3(was)-275.9(low.)-272.7(Any)-274.9(increases)-273.5(were)-278.3(normal)-274.1(and)]TJ 0 -1.1174 TD [(constant)-281.9(until)-282.4(they)-282.2(reach)-279.7(stable)-285.5(levels)-285.5(as)-279.6(shown)-282.7(in)-278.8(Table)-279.9(1.)]TJ /F2 1 Tf 8.9663 0 0 8.9663 310.8472 83.9622 Tm [(Molecular)-331(diagnostics)]TJ /F5 1 Tf 9.843 0 0 9.843 320.8251 67.5212 Tm [(Predisinfection,)-363.4(PCR)-362.3(testing)-360.4(conrmed)]TJ /F6 1 Tf 16.3864 0 TD [(M.)-356.9(haemophilum)]TJ /F5 1 Tf -17.4001 -1.1116 TD [(presence)-347.3(in)-336.4(both)-344.4(the)-344.4(tested)-343.1(biolm)-342(and)-344.3(frozen)-342.9(sh)-342.8(samples)]TJ /F4 1 Tf 8.9663 0 0 8.9663 59.7543 749.7069 Tm [(ERADICATION)-340.4(OF)]TJ /F13 1 Tf 8.9975 0 TD (M)Tj 6.4556 0 0 6.4556 147.9118 749.7069 Tm [(YCOBACTER)-14.6(IUM)]TJ /F4 1 Tf 8.9663 0 0 8.9663 540.0565 749.7069 Tm (83)Tj ET endstream endobj 74 0 obj <>stream /GS1 gs BT /F5 1 Tf 9.843 0 0 9.843 62.022 543.6849 Tm 0 0 0 rg 0 Tc 0 Tw [(\(Table)-217.6(2\).)]TJ /F6 1 Tf 4.1355 0 TD [(M.)-218.7(chelonae)]TJ /F5 1 Tf 5.1377 0 TD [(was)-218.3(present)-217.3(in)-215.4(the)-217.7(biolm)-215.3(sample,)-215.1(but)]TJ -9.2731 -1.1174 TD [(not)-308.7(in)-307.6(the)-309.9(sh)-308.2(sample,)-313(and)-309.7(all)-310(tested)-308.5(samples)-310.5(were)-312.8(negative)]TJ 0 -1.1116 TD [(for)-279.7(all)-281.2(the)-281.1(other)]TJ /F6 1 Tf 6.5891 0 TD (Mycobacterium)Tj /F5 1 Tf 6.5718 0 TD (species.)Tj -12.1472 -1.1116 TD [(Postdisinfection)-388.6(\(after)-383.3(6)-387(months\))-383.2(PCR)-385.3(results)-383.1(conrmed)]TJ -1.0137 -1.1174 TD [(that)-236.2(artemia)-237.3(\(live)-237.3(food)-234.8(sample\),)-239.2(water,)-236.9(and)-240.6(sh)-233.3(samples)-235.6(were)]TJ 0 -1.1116 TD [(negative)-404.2(for)]TJ /F6 1 Tf 5.3796 0 TD [(A.)-397.4(hydrophila)]TJ /F5 1 Tf 6.0592 0 TD [(and)-401.9(all)]TJ /F6 1 Tf 3.2658 0 TD (Mycobacterium)Tj /F5 1 Tf 6.6928 0 TD (species)Tj -21.3973 -1.1116 TD [(tested,)-818.8(including)]TJ /F6 1 Tf 8.0175 0 TD [(M.)-817.7(haemophilum)]TJ /F5 1 Tf 7.3494 0 TD [(.)-815.5(Dry)-816.3(food)-816.5(sample)]TJ -15.3669 -1.1174 TD [(\(ZM400\),)-197.5(howev)-7.5(er,)-196.8(tested)-199.1(positive)-200.2(for)]TJ /F6 1 Tf 15.292 0 TD [(A.)-195.8(hydrophila)]TJ /F5 1 Tf 5.4602 0 TD [(.)-193.5(I)0(n)-198(more)]TJ -20.7522 -1.1116 TD [(detail,)-462(both)-459.5(Z-hab)-457.1(systems,)-462.3(water,)-461.5(and)-459.5(sh)-458(samples)-460.3(were)]TJ T* [(negative)-381.2(for)-383.4(all)-379.1(the)-379(tested)]TJ /F6 1 Tf 11.0529 0 TD (Mycobacterium)Tj /F5 1 Tf 6.6697 0 TD [(species)-385.3(together)]TJ -17.7226 -1.1116 TD [(with)-426(all)-430.9(the)-430.8(other)-427.2(tested)-429.5(species,)-429.1(including)]TJ /F6 1 Tf 18.3562 0 TD [(A.)-426.2(hydrophila)]TJ /F5 1 Tf 5.6848 0 TD (,)Tj /F6 1 Tf -24.041 -1.1174 TD [(Flavobacterium)-700.8(columnare)]TJ /F5 1 Tf 11.3812 0 TD (,)Tj /F6 1 Tf .9446 0 TD [(Pseudocapillaria)-700.9(tomentosa)]TJ /F5 1 Tf 11.7152 0 TD (,)Tj /F6 1 Tf -24.041 -1.1116 TD [(Pseudoloma)-215.8(neurophilia)]TJ /F5 1 Tf 9.8376 0 TD (,)Tj /F6 1 Tf .4608 0 TD [(and)-210.7(Pseudomonas)-210.8(aeruginosa)]TJ /F5 1 Tf 11.9687 0 TD [(.)-210.8(The)]TJ -22.267 -1.1116 TD [(biolm)-393.8(samples)-396.9(alone)-393.9(tested)-400.7(positive)-390.3(for)]TJ /F6 1 Tf 17.3482 0 TD [(M.)-391.5(chelo)-8(nae)]TJ /F5 1 Tf 5.4948 0 TD (and)Tj -22.843 -1.1174 TD [(were)-284(negative)-283.3(for)-279.7(all)-281.2(other)-283.2(tested)-285.5(species.)]TJ 1.0137 -1.1116 TD [(Second)-417.7(postdisinfection)-417.6(\(after)-417.9(1)-415.8(year\))-416.7(PCR)-414.1(results)-417.6(con-)]TJ -1.0137 -1.1116 TD [(rmed)-462.7(that)]TJ /F6 1 Tf 5.0513 0 TD [(A.)-460.7(hydrophila)]TJ /F5 1 Tf 6.1859 0 TD (and)Tj /F6 1 Tf 1.9065 0 TD [(M.)-460.6(haemo)-7.5(philum)]TJ /F5 1 Tf 7.4588 0 TD [(were)-462.6(not)]TJ -20.6025 -1.1174 TD [(present)-332.5(in)-324.9(all)-333(the)-327.1(tested)-331.5(samples.)-331.2(For)-331.4(this)-327(test,)-331.6(pooled)-324.7(envi-)]TJ 0 -1.1116 TD [(ronmental)-491.8(samples)-494.8(were)-491.4(used)-489.2(to)-491.9(cover)-491.6(a)-491.8(wider)-491.5(range)-491.6(of)]TJ T* [(testing)-227.9(sites)-225.4(and)-229.1(areas)-227.6(of)-226.8(the)-223.5(systems.)-232(The)-224.5(test)-230.3(was)-224(tailored)-230.4(to)]TJ 0 -1.1174 TD [(specically)-304.8(test)-305.1(just)-304(for)]TJ /F6 1 Tf 9.7857 0 TD [(A.)-305.2(hydrophila)]TJ /F5 1 Tf 5.5639 0 TD (,)Tj /F6 1 Tf .5472 0 TD [(M.)-305.1(chelonae)]TJ /F5 1 Tf 5.0109 0 TD [(,)-302.9(and)]TJ /F6 1 Tf 2.2981 0 TD (M.)Tj -23.2059 -1.1116 TD (haemophilum)Tj /F5 1 Tf 5.4487 0 TD (.)Tj /F6 1 Tf .5529 0 TD [(A.)-305.2(hydrophila)]TJ /F5 1 Tf 5.8691 0 TD (and)Tj /F6 1 Tf 1.7509 0 TD [(M.)-305.1(haemophilum)]TJ /F5 1 Tf 7.1363 0 TD [(were)-307.1(not)]TJ -20.758 -1.1116 TD [(present)-297.9(in)-296.1(any)-298.2(of)-295.9(the)-298.3(tested)-297(environmental)-301.6(samples.)]TJ /F6 1 Tf 21.1842 0 TD [(M.)-293.6(che-)]TJ -21.1842 -1.1116 TD (lonae)Tj /F5 1 Tf 2.229 0 TD [(,)-504.5(however,)-503.9(was)-500.5(present)-505.3(in)-503.4(the)-499.9(tested)-504.3(environmental)]TJ -2.229 -1.1174 TD [(samples,)-290.9(as)-285.4(it)-290.4(was)-287.4(6)-283.3(months)-290(previously.)-286.4(The)-287.9(pool)-286.8(of)-290.2(biolm)]TJ 0 -1.1116 TD [(and)-246.4(sludge)-243.9(both)-246.4(tested)-245.1(positive)-246.3(for)]TJ /F6 1 Tf 13.9961 0 TD [(M.)-247.5(chelonae)]TJ /F5 1 Tf 5.1953 0 TD [(and)-246.4(negative)]TJ -19.1913 -1.1116 TD (for)Tj /F6 1 Tf 1.4457 0 TD [(A.)-282.2(hydrophila)]TJ /F5 1 Tf 5.8231 0 TD (and)Tj /F6 1 Tf 1.7279 0 TD [(M.)-282.1(haemophilum)]TJ /F5 1 Tf 7.0902 0 TD [(\(Table)-286.7(3\).)]TJ /F4 1 Tf 8.9663 0 0 8.9663 313.0582 544.3085 Tm (Discussion)Tj /F5 1 Tf 9.843 0 0 9.843 323.0361 527.8676 Tm [(The)-552.8(disinfection)-551.5(protocol)-551.6(described)-554.7(in)-543.7(this)-551.6(study)-546.9(was)]TJ -1.0137 -1.1174 TD [(successful)-353.8(for)-348.8(system)-348.6(eradication)-351.2(of)-342(the)-350.2(pathogens)]TJ /F6 1 Tf 21.0805 0 TD [(M.)-351.2(hae-)]TJ -21.0805 -1.1116 TD (mophilum)Tj /F5 1 Tf 4.3255 0 TD (and)Tj /F6 1 Tf 1.7682 0 TD [(A.)-328.3(hydrophila)]TJ /F5 1 Tf 5.5869 0 TD [(.)-320.2(A)-324.5(previous)-326.7(disinfection)-326.9(study)]TJ -11.6807 -1.1116 TD [(by)-272.9(Whipps)]TJ /F6 1 Tf 4.6538 0 TD [(et)-274.2(al.)]TJ /F5 1 Tf 7.3823 0 0 6.5614 378.8787 499.351 Tm (11)Tj 9.843 0 0 9.843 388.9133 494.9857 Tm [(used)-270.3(1000)-171.4(ppm)-269.5(bleach)-269.4(\(buffered)-277.1(to)-267.3(pH)-268(7\))]TJ -7.7065 -1.1116 TD [(to)-370.9(sanitize)-369.6(a)-365.1(contaminated)-370.8(aquatic)-368.6(housing)-364.8(system)-371.6(after)-365(an)]TJ 0 -1.1174 TD [(outbreak)-241.6(of)]TJ /F6 1 Tf 4.8209 0 TD [(M.)-236(haemophilum)]TJ /F5 1 Tf 6.7677 0 TD [(.)-233.8(Postdisinfection)-238.9(diagnostics)-239.1(in)]TJ -11.5885 -1.1116 TD [(that)-455.1(study)-454.7(also)-449(revealed)-457.1(that)-455.1(the)-448.1(pathogen)-454.8(was)-454.4(no)-451.5(longer)]TJ T* [(present,)-301.4(but)-291.4(this)-298.2(study)-293.4(lacked)-298.2(step-by-step)-297.6(details)-298.2(of)-290.1(the)-298.3(full)]TJ 0 -1.1174 TD [(disinfection)-384.5(process)-378.2(\(such)-375.1(as)-377.5(contact)-374.3(time)-375.7(with)-379.9(bleach)-378.8(and)]TJ 0 -1.1116 TD [(method)-187.7(of)-180.7(cleaning\).)-184.1(In)-186.5(another)-182.9(study,)-181.7(useful)-186.2(information)-181.9(was)]TJ T* [(report)-7.8(ed)-210.7(regarding)-219.7(the)-211.9(efcacy)-219.5(of)-209.5(bleach)-217.6(against)]TJ /F6 1 Tf 19.3929 0 TD [(M.)-213(marinum)]TJ /F5 1 Tf -19.3929 -1.1174 TD [(after)-301.6(trialing)-298.4(different)-300.4(conta)-8(ct)-297.2(times)-296(and)-298.2(concentrations.)]TJ 7.3823 0 0 6.5614 535.8046 411.7038 Tm (14)Tj 9.843 0 0 9.843 546.1227 407.2818 Tm (It)Tj -23.6781 -1.1116 TD [(was)-454.4(demonstrated)-455.7(that)-449.3(sodium)-451.3(hypochlorite,)-451.3(even)-450.2(at)-452.7(con-)]TJ T* [(centrations)-499.6(as)-492.7(low)-488.1(as)-492.7(200)-164.6(ppm,)-491.8(was)-494.7(effective)-498.6(to)-486.1(stop)]TJ /F6 1 Tf 23.2116 0 TD (M.)Tj -23.2116 -1.1174 TD [(marinu)-7.4(m)]TJ /F5 1 Tf 3.8878 0 TD [(growth)-284(within)-278.6(a)-284.5(60-min)-278.6(contact)-282.2(time.)]TJ 7.3823 0 0 6.5614 499.8613 378.8219 Tm (14)Tj 9.843 0 0 9.843 323.0361 363.4582 Tm [(Using)-455.6(this)-459.5(information)-458.3(and)-453.7(knowledge)-456.7(of)-457.2(other)-456(system)]TJ -1.0137 -1.1116 TD [(saniti)-8(zing)-299.4(examples,)-304.9(we)-280.7(de)19.7(vi)20.7(se)19.9(d)-294.8(a)-290.2(pr)20.9(ot)20.7(oc)19.7(ol)-284.6(im)19.5(pl)20.7(em)18.5(en)19.7(ti)20.6(ng)-278.7(an)]TJ T* [(ac)12.8(ti)20.6(ve)-469.9(ch)19.7(lo)20.7(ri)20.7(ne)-469.9(co)19.7(nc)19.7(en)19.7(tr)20.7(at)13.8(io)20.7(n)-479.1(o)0(f)-468.7(2)0(5)20.8(0)0()20.8(3)0(0)20.8(0)-156.6(pp)20.8(m)-480.4(o)0(v)20.8(e)0(r)-469.9(a)-480.3(pr)20.9(o-)]TJ 0 -1.1174 TD [(lo)15(ng)20.8(ed)-274.1(co)19.7(nt)20.7(ac)12.8(t)-283.5(t)0(i)20.6(m)0(e)18.5(,)-285.7(re)19.7(pe)19.7(at)19.5(in)15(g)-283.3(t)0(h)20.7(e)-284.5(pr)15.1(oc)19.7(es)19.9(s)-283.1(s)0(e)14.1(v)0(e)19.7(r)0(a)19.7(l)-283.5(ti)14.8(m)15(e)0(s)-279.6(w)0(i)19.8(t)0(h)]TJ 0 -1.1116 TD [(an)13.9(d)-283.3(w)0(i)19.8(t)0(h)15(o)0(u)20.8(t)-289.2(wa)18.8(te)19.6(r)-289(m)15(ov)15.1(em)18.5(en)19.7(t)-283.5(t)0(h)15(r)0(o)20.9(u)0(g)20.8(h)-289.1(th)20.7(e)-290.2(s)0(y)21(s)0(t)20.9(e)0(m)18.4(.)-285.7(Th)19.6(e)-290.2(u)0(s)21(e)-290.2(of)]TJ T* [(Cl)13.9(ea)18.5(nl)20.7(in)20.7(e)-405.4(C)0(h)19.8(l)0(o)20.7(r)0(i)20.7(n)0(e)-395(t)0(a)19.6(b)0(l)20.7(e)0(t)19.5(s)-404.1(pr)20.9(ov)20.8(ed)-395(ad)19.7(va)19.7(nt)20.7(ag)19.7(eo)19.7(us)-393.7(as)-394.8(th)20.7(ey)-395(ef)19.7(-)]TJ 0 -1.1174 TD [(fe)14(rv)20.9(es)19.9(ce)-995.2(wi)19.8(th)-987.2(an)-994(ac)18.5(ti)20.6(ve)-994(in)20.7(gr)20.9(ed)19.7(ie)13.8(nt)-987.2(of)-992.8(So)21(di)20.7(um)-994.1(di)20.7(-)]TJ 0 -1.1116 TD [(ch)13.9(lo)20.7(ro)20.9(is)20.9(oc)19.7(ya)19.7(nu)15.1(ra)19.7(te)-256.9(\(w)20(it)20.6(h)-266(a)-267.2(pH)-262.3(of)-255.6(di)20.7(lu)20.7(te)19.6(d)-271.8(s)0(o)21(l)0(u)20.7(t)0(i)20.6(o)0(n)20.8(/)0(1)-146.3(L)0()18.6(5)0(.)18.5(0)-266(to)]TJ T* [(6.)18.5(0\))-370.8(as)-366(an)-366.2(al)19.5(te)19.6(rn)15.1(at)19.5(iv)20.7(e)-376.6(f)0(o)20.9(r)-375.4(so)15.3(di)20.7(um)-366.3(hy)20.8(po)20.8(ch)19.7(lo)15(ri)20.7(te)19.6(.)]TJ 7.3823 0 0 6.5614 499.6912 280.1763 Tm (26)Tj 9.843 0 0 9.843 510.7463 275.8109 Tm -.0139 Tc [(Wh)-13.9(en)-389.4(d)-13.9(i)6.8(s)-13.9(-)]TJ -20.0841 -1.1174 TD 0 Tc [(so)15.3(lv)20.7(ed)-446.9(in)-440.1(wa)18.8(te)13.8(r,)-442.2(th)20.7(es)19.9(e)-457.3(t)0(a)19.5(b)0(l)20.7(e)0(t)19.5(s)-455.9(pr)20.9(ov)15.1(id)20.7(e)-451.5(a)-457.3(me)18.4(as)19.9(ur)20.9(ed)-446.9(do)20.8(se)-446.7(of)]TJ 0 -1.1116 TD [(av)13.9(ai)19.5(la)19.6(bl)20.7(e)-324.8(c)0(h)13.9(l)0(o)20.7(r)0(i)20.7(n)0(e)19.7(,)-326(wh)20(ic)19.6(h)-329.4(c)0(a)18.5(n)-329.4(be)-314.4(us)21(ed)-320.1(fo)20.9(r)-329.4(t)0(h)20.7(e)-330.6(di)20.7(si)20.9(nf)20.9(ec)18.5(ti)20.6(on)-319(of)]TJ T* [(eq)13.9(ui)20.7(pm)19.6(en)19.7(t,)-344.5(wo)20(rk)-347.7(su)21(rf)20.9(ac)18.5(es)19.9(,)-354.8(a)0(n)19.7(d)-358.2(ho)20.8(ld)20.7(in)20.7(g)-358.2(s)0(y)21(s)0(t)20.9(e)0(m)18.5(s)0(.)-344.2(S)0(o)21(d)0(i)14.9(u)0(m)-343.2(d)0(i)20.7(-)]TJ T* [(ch)13.9(lo)20.7(ro)20.9(is)20.9(oc)19.7(ya)19.7(nu)15.1(ra)19.7(te)-187.8(ha)19.7(s)-196.7(a)-192.3(hi)14.9(gh)-180.7(an)13.9(d)-191.2(r)0(a)14(p)0(i)20.7(d)-196.9(ac)18.5(ti)20.6(vi)20.7(ty)-186.6(ag)19.7(ai)19.5(ns)21(t)-197.1(a)-198.1(wi)19.8(de)]TJ 0 -1.1174 TD [(ra)14(ng)20.8(e)-267.2(o)0(f)-261.4(b)0(a)19.7(c)0(t)19.5(e)0(r)13.9(i)0(a)19.6(,)-268.4(fu)20.9(ng)20.8(i,)-263.8(sp)21(or)20.9(es)19.9(,)-274.1(a)0(n)19.7(d)-266(vi)20.7(ru)15.1(se)19.9(s.)]TJ 7.3823 0 0 6.5614 485.1211 225.3543 Tm -.0173 Tc [(26)-17.3(,27)]TJ 9.843 0 0 9.843 323.0361 210.0472 Tm 0 Tc [(Cleanline)-222.2(Tablets)-224.4(also)-218.6(offer)-219.7(economic)-221(advantages)-222.9(in)-221.2(terms)]TJ -1.0137 -1.1116 TD [(of)-272.9(ease)-272.6(of)-267.1(use,)-272.6(accurate)-273.9(dilution,)-271.9(and)-275.2(prolonged)-270.4(shelf)-271.5(life.)-268.4(In)]TJ 0 -1.1174 TD [(relation)-259.2(of)-255.6(cost-effectiveness)-262.7(for)-250.9(a)-255.7(stand-alone)-257.5(unit,)-255.8(which)-255.3(is)]TJ 0 -1.1116 TD [(300350)-169(L)-238.4(total)-243.2(volume,)-243(it)-238.6(is)-238.3(necessary)-244.5(to)-238.5(use)-246.2(approximately)]TJ T* [(half)-230.3(of)-221(a)-226.9(bottle)-227(of)-221(tablets)-229.1(\(around)-227.7(7080)-224.4(tablets)-229.1(per)-223.3(unit\).)-228(The)]TJ 0 -1.1174 TD [(efcacy)-444.1(of)-434.1(Cleanline)-441(Chlorine)-437.5(tablets)-442.2(alone)-439.9(had)-436.4(not)-435.4(been)]TJ 0 -1.1116 TD [(tested)-331.5(before)-331.4(the)-327.1(use)-332.6(of)-324.7(Virkon)-330.1(Aquatic,)-331.4(but)-326(previous)-332.4(work)]TJ T* [(has)-390.2(shown)-386.3(that)-386(Virkon)]TJ /F7 1 Tf 7.3823 0 0 6.5614 405.9778 137.707 Tm ()Tj /F5 1 Tf 9.843 0 0 9.843 415.6156 133.3417 Tm [(alone)-388.1(is)-388(ineffective)-385.7(for)-389.1(the)-384.7(elimi-)]TJ -10.4193 -1.1174 TD [(nation)-209.6(of)-203.8(certain)]TJ /F6 1 Tf 6.6985 0 TD (Mycobacterium)Tj /F5 1 Tf 6.4969 0 TD (spp.)Tj 7.3823 0 0 6.5614 459.0424 126.7653 Tm (14,28)Tj 9.843 0 0 9.843 477.6944 122.3433 Tm [(It)-203.9(was)-206.7(possible)-205.7(that)]TJ -16.7262 -1.1116 TD [(the)-344.4(original)-344.4(wild-origin)-341.7(population)-340.9(carried)-345.3(additional)-342.2(unde-)]TJ T* [(tecte)-9.3(d)-191.2(pathogens,)-194.2(including)-195.8(other)-191.1(bacterial)-199.2(or)-192.2(viral)-197(species,)-192.9(so)]TJ T* [(it)-307.7(was)-304.6(decided)-310.8(that)-305.3(Virkon)-307.1(Aquatic)-310.7(would)-306(be)-302.9(used)-304.9(as)-308.4(a)-307.5(sec-)]TJ 0 -1.1174 TD [(ondary)-399.4(disinfectant)-397.1(agent)-393.9(in)-394(our)-389.2(disinfection)-396(method.)-398.5(This)]TJ 0 -1.1116 TD [(product)-435.2(was)-437.1(chosen)-435(due)-436.4(to)-428.5(its)-435.3(high)-430.8(range)-439.8(of)-428.4(antibacterial)]TJ T* [(and)-603.5(antiviral)-606(effects,)-602(and)-603.5(there)-601.2(are)-604.6(a)-601.3(range)-601(of)-601.2(general)]TJ ET 62.022 709.512 490.167 .22681 re f 62.022 634.223 490.167 .28351 re f 219.685 690.633 84.983 .22668 re f 343.446 690.633 84.983 .22668 re f 467.206 690.633 84.983 .22668 re f 62.022 672.945 490.167 .22681 re f BT /F9 1 Tf 9.845 0 0 9.845 106.7527 725.1588 Tm (Table)Tj /F5 1 Tf 3.0002 0 TD (2.)Tj /F9 1 Tf 1.2496 0 TD [(Summary)-332(of)-337(Bacterial)-332.2(Presence)-337(in)-331.6(the)-332.4(Zebrash)-339(Unit,)-332(Before)-336.2(and)-332(Six)-336.8(Months)]TJ 6.1329 -1.0135 TD [(After)-335.2(the)-332.4(Described)-337.8(Disinfection)-333.1(Protocol)]TJ /F6 1 Tf -14.9261 -3.6567 TD (Species)Tj 18.8823 1.8139 TD [(Biolm)-9901.5(Water)-10458.9(Fish)]TJ -2.8678 -1.8139 TD [(Before)-3948.3(After)-3940.6(Before)-3948.3(After)-3940.6(Before)-3948.3(After)]TJ -16.0145 -1.9003 TD [(Mycobacterium)-342(haemophilum)]TJ /F5 1 Tf 16.7113 0 TD [(2/2)-5007.3(0/2)-4926.7(ND)-4927.2(0/2)-5007.3(2/2)-5001.6(0/2)]TJ /F6 1 Tf -16.7113 -1.0077 TD [(Mycobacterium)-342(chelonae)]TJ /F5 1 Tf 16.7113 0 TD [(2/2)-5007.3(2/2)-4926.7(ND)-4927.2(0/2)-5007.3(0/2)-5001.6(0/2)]TJ /F6 1 Tf -16.7113 -1.0135 TD [(Aeromonas)-334.2(hydrophila)]TJ /F5 1 Tf 16.6364 0 TD [(ND)-4927.2(0/2)-4926.7(ND)-4927.2(0/2)-4926.7(ND)-4927.2(0/2)]TJ 8.7511 0 0 8.7511 70.0157 622.4313 Tm [(After)-479.4(a)-479.4(mycobacterium-positive)-482.9(histopathology)-477(report)-475.9(on)-483.5(sh)-480.6(samples,)-475.8(a)-479.4(customized)-484.1(polymerase)-477.5(chain)-481.1(reaction)-482.6(\(PCR\))-481.9(panel)-481.1(was)]TJ -.9134 -1.0236 TD [(performed)-445.1(to)-449.9(identify)-446.6(species.)-447(Postdisinfection,)-448.1(a)-447(circulum)-446.6(sampling)-445.3(PCR)-448.2(panel)-448.7(was)-445.3(used)-448.4(for)-447.1(full)-444.9(system)-447.2(diagnostics.)]TJ /F6 1 Tf 50.311 0 TD [(A.)-444.6(hydrophila)]TJ /F5 1 Tf -50.311 -1.0236 TD [(presence)-322.7(in)-320.3(the)-320.3(system)-324.1(and)-321.6(sh)-318.6(was)-322.3(conrmed)-319.4(by)-321.6(histology)-314.5(and)-321.6(bacteriology)-321.2(test,)-318.9(but)-325.6(was)-315.8(not)-325.6(included)-320.9(in)-320.3(the)-320.3(predisinfection)-319.9(PCR)-318.6(panel)]TJ T* [(test,)-331.9(only)-337.4(in)-333.3(the)-333.3(postdisinfection)-329(testing.)]TJ .9134 -1.0301 TD [(Zero)-334.6(\(0\))-330.5(represents)-334.3(a)-336.9(negative)-328.6(result)-338.4(for)-330.5(the)-333.3(tested)-334.6(pathogen.)]TJ 0 -1.0236 TD [(ND,)-328.8(no)-334.5(data)-333.3(were)-335.4(present)-333.2(on)-334.5(PCR)-331.6(test)-335.7(\(not)-329.5(tested)-334.6(by)-334.5(PCR\).)]TJ ET 62.022 179.546 239.131 .28342 re f 62.022 122.173 239.131 .28348 re f 62.022 160.894 239.131 .22676 re f BT /F9 1 Tf 9.845 0 0 9.845 94.7905 215.1495 Tm (Table)Tj /F5 1 Tf 3.0002 0 TD (3.)Tj /F9 1 Tf 1.2496 0 TD [(Results)-337(of)-313.9(Ba)20.2(ct)21.1(er)21.1(ia)21(l)-323.5(T)0(e)19.9(s)0(t)15.5(i)0(n)19.7(g)]TJ -4.1807 -1.0135 TD [(in)-314.3(th)19.9(e)-323.5(Z)15.1(eb)15.3(ra)21.2(s)20.4(h)-324.6(U)0(n)19.1(i)0(t)-313.1(O)0(n)19.1(e)-323.5(Ye)20.2(ar)-312.8(Af)19.1(te)21.1(r)]TJ -2.8505 -1.0135 TD [(th)19.9(e)-323.5(D)0(i)14.3(s)0(i)21(n)0(f)18.7(e)0(c)21.1(t)0(a)21.2(n)0(t)-314.1(P)0(r)21.2(o)0(t)21.2(o)0(c)15.5(o)0(l)-312.8(W)14.1(as)-318.4(Im)20.1(pl)19.9(em)20.1(en)19.9(te)21.1(d)-323.3(\()0(H)20.2(a)0(l)21.2(f)]TJ .5586 -1.0077 TD [(a)-323.3(Y)0(e)20.2(a)0(r)-312.8(A)0(f)13.3(t)0(e)21.1(r)-323.5(Fi)21.1(rs)21.2(t)-323.5(M)0(o)20.4(l)0(e)21.1(c)0(u)19.9(l)0(a)21.2(r)-323.5(Di)14.3(ag)21.4(no)20.1(st)21.2(ic)20.9(s)-323.3(T)0(e)19.9(s)0(t)15.5(\))]TJ /F6 1 Tf -1.1056 -1.8427 TD [(Species)-8757.7(Biolm)-5899.3(Sludge)]TJ 0 -1.9003 TD [(M.)-333.6(haemophilum)]TJ /F5 1 Tf 10.8491 0 TD [(Not)-332.2(present)-3982.7(Not)-338(present)]TJ /F6 1 Tf -10.8491 -1.0077 TD [(M.)-333.6(chelonae)]TJ /F5 1 Tf 10.8491 0 TD [(Present)-5761.9(Present)]TJ /F6 1 Tf -10.8491 -1.0135 TD [(A.)-333.8(hydrophila)]TJ /F5 1 Tf 10.8491 0 TD [(Not)-332.2(present)-3982.7(Not)-338(present)]TJ 8.7511 0 0 8.7511 70.0157 110.3811 Tm [(For)-545.6(each)-556(type)-552.4(of)-546.9(sample)-553.7(\(biolm)-548.9(or)-553.4(sludge\),)-548.5(ve)-548.2(individual)]TJ -.9134 -1.0236 TD [(samples)-252.9(were)-257.7(collected)-256(\(5)]TJ /F7 1 Tf 10.5662 0 TD ()Tj /F5 1 Tf .7191 0 TD [(biolm,)-249.7(5)]TJ /F7 1 Tf 4.0619 0 TD ()Tj /F5 1 Tf .7191 0 TD [(sludge,)-253.1(10)-250.3(samples)-259.4(in)-249.1(total\).)]TJ -16.0663 -1.0236 TD [(The)-544.5(samples)-544.4(were)-542.7(pooled)-543.6(into)-543.5(a)-544.2(combined)-540(test)-543(sample)-547.2(by)-541.9(the)]TJ 0 -1.0301 TD [(diagnostics)-384.2(company)-380.5(\(combining)-378.3(the)-385.1(ve)-379.7(biolm)-383.1(samples)-382.5(for)-382.3(one)]TJ 0 -1.0236 TD [(test)-413.5(and)-412.3(the)-404.6(ve)-412.1(sludge)-412.4(tests)-410.7(as)-411.8(another\))-410(for)-414.7(PCR.)-405.5(As)-412.9(this)-412.3(was)-413(a)]TJ T* [(pooled)-239.1(test)-238.6(of)-235.9(all)-241.3(ve)-237.2(samples,)-242.6(pathogens)-235.2(are)-237.1(deemed)-240.3(as)-243.4(being)-233.8(either)]TJ T* [(present)-333.2(or)-333.1(not)-332.1(present.)]TJ /F4 1 Tf 8.9663 0 0 8.9663 62.022 749.6502 Tm (84)Tj 40.846 0 TD (RA)Tj .9168 .2087 TD ()Tj .5185 -.2087 TD [(CZ,)-334.5(DWYER,)-341.5(AND)-330.9(KILLEN)]TJ ET endstream endobj 78 0 obj <>stream /GS1 gs BT /F5 1 Tf 9.843 0 0 9.843 59.7543 725.1588 Tm 0 0 0 rg 0 Tc 0 Tw [(recommendation)-11.3(s)-432.9(for)-429.5(its)-435.3(use)-436.2(in)-428.5(aquaculture)-437.5(as)-435.1(a)-434.2(safe)-432.7(and)]TJ 0 -1.1174 TD [(harmless)-288.5(treatment)-279.9(for)-285.5(sh.)]TJ 7.3823 0 0 6.5614 168.6614 718.5825 Tm (29)Tj 9.843 0 0 9.843 69.7323 703.2188 Tm [(Following)-316.3(the)-315.6(histopathology)-322.2(reports)-314(which)-318.7(indicated)-319.1(se-)]TJ -1.0137 -1.1116 TD [(vere)-663.3(systemic)-663.1(mycobacterial)-666.8(infection,)-661.1(the)-661.2(presence)-664.1(of)]TJ /F6 1 Tf T* (Aeromonas)Tj /F5 1 Tf 4.9188 0 TD [(species)-368(w)15.3(a)15(s)-346.5(t)16.1(h)16.2(o)0(u)26.6(g)16.2(h)16.2(t)-346.8(to)-336.4(b)16.2(e)-347.8(a)-347.8(s)16.4(e)15(c)15(o)0(n)26.6(d)16.2(a)15(r)16.2(y)-352.4(i)16.1(n)16.2(f)16.2(e)15(c)15(t)0(i)26.3(o)16.2(n)]TJ -4.9188 -1.1174 TD -.0159 Tc [(c)-15.9(a)8.4(used)-230.1(by)-230.1(the)-231.3(m)-.9(ycoba)-15.9(c)8.4(t).2(eri)-15.9(a)-215.2(su)-15.9(p)10.7(pres)-15.9(s)11.1(ing)-230.1(t).2(he)-231.3(immun)-15.9(e)-215.1(s)-15.9(y)10.9(stem)-231.3(of)]TJ 0 -1.1116 TD -.0158 Tc [(t)-15.8(h)10.7(e)-277.2().5(sh.)-278.4(T)-.8(heref)-15.8(o)10.8(re,)-278.4(our)-276.1(atte)-15.8(n)9.6(tion)-276.1(f).4(o)-15.8(c)9.6(used)-276.1(on)-276.1(the)-277.2(e)-.8(r)-15.8(a)9.7(dicat)-15.8(i)10.5(o).4(n)-276.1(o).4(f)]TJ T* -.0156 Tc [(p)-15.6(r)11(imary)-247.1(m)-.6(y)-15.6(c)9.8(obact)-15.6(e)9.7(r).6(ial)-247.2(p).6(r)-15.6(e)9.9(senc)-15.6(e)-239(r).6(ather)-247.1(t)-15.6(h)10.9(a)-.6(n)-247.1(t).5(hat)-247.2(o)-15.6(f)]TJ /F6 1 Tf 19.8652 0 TD -.0158 Tc [(Aeromo)-15.8(n)10.8(as)]TJ /F5 1 Tf -19.8652 -1.1174 TD [(s)-15.8(p)11(ecie)-15.8(s)9.8(.)-359(T)-.8(he)-363.6(pre)-15.8(s)9.8(e)-.8(nce)-363.6(o).4(f)]TJ /F6 1 Tf 10.1774 0 TD -.0156 Tc [(Aer)-15.6(o)11.2(m)-.3(ona)-15.6(s)]TJ /F5 1 Tf 4.7748 0 TD [(s)-15.5(p)11.3(ecies)-15.5(,)-348.1(how)-15.5(e)9.1(v).7(er,)-364.5(d).7(em-)]TJ -14.9522 -1.1116 TD -.016 Tc [(o)-16(n)10.6(str)-16(a)9.5(ted)-489.4(t)-16(h)10.5(e)-490.6(lik)-16(e)9.4(liho)-16(o)10.6(d)-489.4(o).2(f)-495.1(m)-1(ultis)-16(e)9.6(c)-1(ond)-16(a)9.4(r).2(y)-489.4(i).1(n)-16(f)10.6(ecti)-16(o)10.5(n)-489.4(a)-1(ge)-16(n)9.4(t).1(s)]TJ T* -.0158 Tc [(b)-15.8(e)9.6(ing)-356.7(pres)-15.8(e)9.8(nt)-356.8(in)-356.7(the)-357.9(s).6(yst)-15.8(e)9.5(m,)-353.3(as)-356.5(wel)-15.8(l)-340.8(as)-356.5(th)-15.8(e)-341.7(h).4(i)-15.8(g)10.7(h)-356.7(l).3(oad)-356.7(o).4(f)-356.7(m)-.8(y-)]TJ 0 -1.1174 TD -.0159 Tc 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[(progress)-389.7(was)-391(regularly)-386.8(checked)-392.5(as)-389.1(in)-382.5(step)-391.4(10)-382.3(of)-388.1(the)-390.5(disin-)]TJ 0 -1.1174 TD [(fection)-188.8(protocol.)-186.4(For)-181.7(the)-183.1(following)-183.9(7)-185.4(months)-180.6(after)-186.4(tagging)-181.9(and)]TJ 0 -1.1116 TD [(transferring)-332.3(to)-324.9(the)-321.4(glass)-329(aquarium)-323.5(systems,)-329.9(there)-324.7(have)-329.3(been)]TJ T* [(no)-186.5(unexpected)-182.8(losses)-185.9(or)-180.7(clinical)-191.4(signs,)-181.5(as)-181.7(were)-186.1(seen)-185.1(during)-179.5(the)]TJ 0 -1.1174 TD [(disease)-287.4(outbreak.)]TJ 1.0137 -1.1116 TD [(From)-437.4(an)-435.3(amalgamation)-441.2(of)-434.1(our)-435.2(invest)-7.8(igation)-435.5(results)-434.9(and)]TJ -1.0137 -1.1116 TD [(postdisinfection)-187.2(observations)-188.2(in)-180.9(differing)-184(generations,)-186.1(we)-188.5(can)]TJ 0 -1.1174 TD [(conclude)-329.2(that)-328.4(the)-327.1(system)-325.5(sanitizing)-330.4(protocol)-327(we)-326.8(describe)-329(in)]TJ 0 -1.1116 TD [(this)-234.9(study)-235.8(is)-232.5(sufcient)-236.7(to)-238.5(eradicate)-234.8(both)]TJ /F6 1 Tf 15.845 0 TD [(M.)-236(haemophilum)]TJ /F5 1 Tf 7.0038 0 TD (and)Tj /F6 1 Tf 2.661 67.9184 TD [(A.)-466.5(hydrophila)]TJ /F5 1 Tf 6.1917 0 TD [(from)-466.3(this)-465.2(type)-466.5(of)-468.7(facility.)-464.3(Postdisinfection,)]TJ -6.1917 -1.1174 TD [(newly)-226.5(stocked)-228.8(sh)-221.8(continued)-231.4(to)-227(be)-222.2(healthy)-229.2(for)-227.9(at)-222.4(least)-232.5(1)-225.7(year.)]TJ 0 -1.1116 TD [(Additional)-439.8(evidence)-445.6(for)-435.2(the)-442.3(absence)-444.1(of)-439.9(these)-439.7(species)-442.9(was)]TJ T* [(provided)-401.7(by)-393.9(negat)-8(ive)-396.3(results)-400.4(in)-399.7(postdisinfection)-406.1(diagnostic)]TJ T* [(tests)-283(for)-279.7(bacterial)-285.6(presence.)]TJ 1.0137 -1.1174 TD [(We)-287.9(believe)-299.4(that)-288.1(the)-292.6(above)-295.8(information,)-294.7(and)-292.4(the)-292.6(presented)]TJ -1.0137 -1.1116 TD 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[(clear)-452.6(outs)-447.8(and)-448(repopulation)-447.9(are)-449.1(constantly)-451.3(occurring.)-453.5(The)]TJ T* [(presented)-243.7(protocol)-240.6(in)-238.5(this)-234.9(study)-241.6(will)-236.1(be)-239.5(useful)-243.8(and)-240.6(benecial,)]TJ 0 -1.1174 TD [(especially)-378.7(for)-383.4(the)-373.2(disinfection)-384.5(of)-376.5(these)-382.1(quarantine)-377.6(units,)-383.4(in)]TJ 0 -1.1116 TD [(every)-278.5(zebra)-9(sh)-279.4(research)-283.9(facility.)]TJ 1.0137 -1.1116 TD [(The)-506.8(simplicity)-509.2(of)-509(this)-505.6(disinfection)-511.2(protocol)-511.3(allows)-509.8(for)]TJ -1.0137 -1.1174 TD [(simple)-383.4(adjustment)-383.4(to)-382.5(be)-377.7(used)-385.5(in)-376.7(different)-386.8(settings,)-382(such)-385.5(as)]TJ 0 -1.1116 TD [(ow-through)-329.7(or)-330.5(recirculation)-336.2(systems)-332.2(\(both)-333.9(glass)-329(and)-332.8(poly-)]TJ T* [(carbonate)-262.3(designs\),)-260.8(and)-257.9(can)-259(be)-256.8(adapted)-259(to)-255.8(cater)-262.5(to)-255.7(smaller)-264.7(or)]TJ 0 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0 9.843 320.8251 387.2692 Tm [(T)15(h)0(e)-205(a)0(u)19.7(t)0(h)20.7(o)0(r)20.9(s)-214(th)20.7(an)19.7(k)-208.4(G)0(r)20(a)0(h)13.9(a)0(m)-200.4(L)0(a)18.5(w)-209.3(an)19.7(d)-208.4(D)0(r)20(.)-210.8(Mi)19.8(ch)19.7(ae)12.8(l)-208.6(W)0(i)18.4(l)0(k)20.7(i)0(n)20.7(s)0(o)21(n)]TJ -1.0137 -1.1116 TD [(fo)20.9(r)-294.8(t)0(h)20.7(e)0(i)19.5(r)-294.8(as)19.9(si)20.9(st)15.1(an)19.7(ce)-281(an)13.9(d)-289.1(M)0(a)18.8(r)0(t)15(i)0(n)-278.8(K)0(e)18.8(n)0(n)15.1(e)0(d)19.7(y)-289.1(of)-284.4(MB)18.9(KI)-285.3(fo)20.9(r)-294.8(a)0(d)19.7(v)0(i)20.7(c)0(e)]TJ 0 -1.1174 TD [(re)19.7(ga)19.7(rd)20.9(in)15(g)-427.3(s)0(y)21(s)0(t)20.9(e)0(m)-419.3(f)0(u)15.1(n)0(c)19.7(t)0(i)20.6(o)0(n)20.8(i)0(n)20.7(g)-433.1(an)19.7(d)-427.3(c)0(h)19.7(e)0(m)18.5(i)0(c)19.5(a)0(l)-424(e)0(x)19.7(p)0(o)20.8(s)0(u)21(r)0(e)19.7(s)0(.)-419(T)0(h)19.6(e)]TJ 0 -1.1116 TD 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[(ba)19.7(ct)19.5(er)19.7(ia)19.5(l)-375.6(i)0(d)20.7(e)0(n)19.7(t)0(i)20.6()0(c)19.8(a)0(t)13.8(i)0(o)20.7(n)-369.7(an)19.7(d)-375.5(f)0(o)20.9(r)-369.7(pr)15.1(ov)20.8(id)20.7(in)20.7(g)-375.5(i)0(n)20.7(f)0(o)20.9(r)0(m)19.7(a)0(t)19.6(i)0(o)20.7(n)-375.5(on)-359.3(di)20.7(-)]TJ T* [(ag)19.7(no)20.8(st)20.9(ic)-280(de)19.7(ta)19.5(il)20.6(s.)-280.8(Th)19.6(e)-284.5(a)0(u)19.7(t)0(h)15(o)0(r)20.9(s)-288.9(al)19.5(so)-272.7(th)15(an)19.7(k)-283.3(t)0(h)20.7(e)-290.2(th)20.7(re)19.7(e)-290.2(a)0(n)19.7(o)0(n)20.8(y)0(m)19.6(o)0(u)20.8(s)]TJ 0 -1.1174 TD [(re)19.7(fe)19.7(re)19.7(es)-279.6(fo)20.9(r)-289.1(t)0(h)20.7(e)0(i)19.5(r)-289(he)19.7(lp)20.7(fu)20.9(l)-289.2(g)0(u)20.8(i)0(d)20.7(a)0(n)19.7(c)0(e)-275.2(a)0(n)13.9(d)-283.3(co)19.7(mm)18.4(en)19.7(ts)-278.6(on)-272.9(th)15(e)-284.5(a)0(r)19.7(t)0(i)20.6(-)]TJ 0 -1.1116 TD [(cl)19.5(e.)-443.4(S.)24.4(S.)18.7(K.)-443.1(is)-439.9(su)15.3(pp)20.8(or)20.9(te)19.6(d)-450.4(b)0(y)-445.7(N)0(a)18.8(t)0(u)20.7(r)0(a)19.7(l)-450.5(En)19.6(vi)20.7(ro)15.1(nm)19.6(en)19.7(t)-450.5(R)0(e)18.6(s)0(e)19.9(a)0(r)19.7(c)0(h)]TJ T* [(Co)19.8(un)20.8(ci)19.6(l)-392.9(A)0(d)20(v)0(a)19.7(n)0(c)19.7(e)0(d)-383.5(F)0(e)19.8(l)0(l)20.6(o)0(w)20(s)0(h)21(i)0(p)-382.5(N)0(E)18.8(/)0(J)20.9(0)0(1)20.8(9)0(1)15.1(0)0(0)20.8(/)0(1)-376.7(a)0(n)13.9(d)-387(Eu)19.6(ro)20.9(pe)19.7(an)]TJ 0 -1.1174 TD [(Re)18.6(se)19.9(ar)19.7(ch)-262.5(Co)19.8(un)20.8(ci)19.5(l)-271.9(s)0(t)20.9(a)0(r)13.9(t)0(i)20.6(n)0(g)-261.4(g)0(r)20.9(a)0(n)19.7(t)-271.9(64)20.8(00)20.8(04)20.8(.)]TJ /F4 1 Tf 8.9663 0 0 8.9663 310.8472 241.1716 Tm [(Disclosure)-336.7(Statement)]TJ /F5 1 Tf 9.843 0 0 9.843 320.8251 224.7307 Tm [(None)-225.3(of)-226.8(the)-229.2(authors)-233.4(of)-226.8(this)-229.1(article)-230.4(has)-228.9(nancial)-231.3(or)-232.6(personal)]TJ -1.0137 -1.1174 TD [(relationship)-436.3(with)-437.5(other)-438.7(people)-441(or)-434.1(organizations)-438.4(that)-437.8(could)]TJ 0 -1.1116 TD [(inappropriately)-285.4(inuence)-283(or)-278.6(bias)-282(the)-281.1(content)-286.8(of)-278.6(the)-281.1(article.)]TJ /F4 1 Tf 8.9663 0 0 8.9663 310.8472 177.222 Tm (References)Tj /F5 1 Tf 9.2951 0 0 9.2951 315.496 161.1779 Tm [(1.)-500.4(Lidster)-379.4(K,)-373.8(Readman)-383.9(GD,)-375.6(Prescott)-384.8(MJ,)-376.9(Owen)-380(SF.)-372.1(Interna-)]TJ 1.2503 -1.1284 TD [(tional)-448.6(survey)-451.8(on)-451.6(the)-449.3(use)-449(and)-449.9(welfare)-458.4(of)-447.8(zebrash)]TJ /F6 1 Tf 21.4693 0 TD (Danio)Tj -21.4693 -1.1223 TD (rerio)Tj /F5 1 Tf 2.3299 0 TD [(in)-335.2(research.)-339(J)-334.7(Fish)-331.4(Biol)-333.6(2017;90:18911905.)]TJ -3.5802 -1.1284 TD [(2.)-500.4(Lawrence)-347(C.)-346.6(The)-348.1(husbandry)-342.4(of)-344.2(zebrash)-347.5(\()]TJ /F6 1 Tf 18.7003 0 TD [(Danio)-343.3(rerio)]TJ /F5 1 Tf 4.8428 0 TD [(\):)-343.6(A)]TJ -22.2927 -1.1223 TD [(review.)-335.9(Aquaculture)-334.1(2007;269:120.)]TJ -1.2503 -1.1284 TD [(3.)-500.4(P)15.3(a)0(v)19.9(l)0(i)19.2(d)0(i)18.6(s)-316.4(M,)-310.5(Di)23.1(gk)18(a)-315.4(N)0(,)-312.9(T)0(h)22.2(e)0(o)19.9(d)0(o)24.1(r)0(i)16.3(d)0(i)-304.7(A)0(,)-312.9(C)13.4(am)20.3(po)-311.3(A,)-306.8(B)13.4(a)0(r)17.6(s)12.9(ak)19.9(is)-309.9(K,)]TJ 1.2503 -1.1223 TD -.019 Tc [(Sk)-19(ou)-19(ra)-19(da)-19(ki)-6.4(s)-384.2(G)-19(,)]TJ /F6 1 Tf 6.5201 0 TD 0 Tc [(et)-351.6(al)18.6(.)]TJ /F5 1 Tf 2.4519 0 TD [(Hu)22.5(sb)18.9(a)14(n)0(d)18(r)0(y)-350.3(o)0(f)-350.3(z)0(e)15.7(b)0(r)21.8(a)14(s)16(h,)]TJ /F6 1 Tf 9.9845 0 TD [(Da)22.5(ni)18.6(o)-360(r)0(e)20.8(r)0(i)19.5(o)]TJ /F5 1 Tf 4.7635 0 TD (,)Tj -23.7199 -1.1284 TD [(a)14(n)0(d)-274.7(t)0(h)18.6(e)-284.9(co)19.9(rt)16.3(i)12.6(s)12.9(ol)-280.3(s)12.9(t)12.6(re)17.6(s)12.9(s)-292(r)15.8(e)0(s)14.7(p)0(o)24.1(n)0(s)18.9(e)14(.)-286.7(Z)0(e)18(b)0(r)21.8(a)0()23.1(s)0(h)-280(2)0(0)24.1(1)0(3)18(;)12.6(10)24.1(:5)18.6(24)24.1(5)18(31)24.1(.)]TJ -1.2503 -1.1223 TD [(4.)-500.4(Nu)]TJ 2.0493 .0122 TD ()Tj .4147 -.0122 TD [(sslein-Volhard)-714.6(C,)-706.4(Dahm)-707.3(R:)-705.9(ZebrashA)-707(Practical)]TJ -1.2137 -1.1284 TD [(Approach.)-355.1(Oxford)-348.6(University)-354.6(Press,)-356.1(Oxford,)-354.8(United)-353.2(King-)]TJ 0 -1.1223 TD [(dom,)-329.4(2002.)]TJ /F4 1 Tf 8.9663 0 0 8.9663 59.7543 749.7069 Tm [(ERADICATION)-340.4(OF)]TJ /F13 1 Tf 8.9975 0 TD (M)Tj 6.4556 0 0 6.4556 147.9118 749.7069 Tm [(YCOBACTER)-14.6(IUM)]TJ /F4 1 Tf 8.9663 0 0 8.9663 540.0565 749.7069 Tm (85)Tj ET endstream endobj 82 0 obj <>stream /GS1 gs BT /F5 1 Tf 9.2951 0 0 9.2951 66.6708 725.1588 Tm 0 0 0 rg 0 Tc 0 Tw [(5.)-500.4(Stewart)-301.7(AM,)-300.1(Braubach)-301.4(O,)-300.7(Spitsbergen)-299.9(J,)-304.3(Gerlai)-298.7(R,)-303.9(Kalueff)]TJ 1.2503 -1.1223 TD [(AV.)-522(Zebrash)-522(models)-521.6(for)-523.5(translational)-525.4(neuroscience)-523.8(re-)]TJ 0 -1.1284 TD [(search:)-459.7(From)-456.7(tank)-455.6(to)-451.1(bedside.)-459.4(Trends)-453.6(Neurosci)-463.4(2014;37:)]TJ 0 -1.1223 TD (264278.)Tj -1.2503 -1.1283 TD [(6.)-500.4(Varga)-476.6(ZM.)-471.2(Aquaculture,)-474.5(husbandry,)-470.6(and)-474.3(shipping)-468.8(at)-473.6(the)]TJ 1.2503 -1.1223 TD [(Zebrash)-253.7(International)-254.7(Resource)-251.8(Center.)-253.8(Methods)-247(Cell)-252.4(Biol)]TJ 0 -1.1284 TD (2016;135:509534.)Tj -1.2503 -1.1223 TD [(7.)-500.4(Kent)-292.5(ML,)-288.2(Feist)-292.5(SW,)-288.2(Harper)-290(C,)-291.7(Hoogstraten-Miller)-293.7(S,)-289.8(Law,)]TJ 1.2503 -1.1284 TD [(Mac)-331.4(J,)-328.7(Sa)]TJ 3.69 .0427 TD ()Tj .3843 -.0427 TD [(nchez-Morgado)-333.7(JM,)]TJ /F6 1 Tf 8.5206 0 TD [(et)-327.2(al.)]TJ /F5 1 Tf 2.4153 0 TD [(Recommendations)-332(for)]TJ -15.0102 -1.1223 TD [(control)-250.4(of)-246.6(pathogens)-252.2(and)-248.6(infectious)-253.5(diseases)-248.4(in)-249.8(sh)-246.4(research)]TJ 0 -1.1284 TD 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[(,)-298.9(a)14(n)18.2(d)-299(o)18.2(t)12.6(h)18.2(e)14(r)-301.3(m)18.6(yc)32.1(ob)30.2(a)14(c)14(t)18.7(e)14(r)15.8(i)18.7(a)-303.2(i)18.7(n)-299(dr)27.9(i)18.7(n)0(k)30.2(i)18.7(ng)-286.9(w)16.6(a)14(t)18.7(e)14(r)]TJ -7.0629 -1.1284 TD [(d)18.2(i)12.6(s)19(t)12.6(r)15.8(i)18.7(bu)30.2(t)18.7(i)12.6(o)18.2(n)-280.7(s)19(y)0(s)31.1(t)12.6(e)20.1(m)12.5(s)19(.)-280.6(A)16.6(p)18.2(pl)-262(E)16.3(n)0(v)30.2(i)12.6(r)15.8(o)18.2(n)-274.6(M)12.8(i)18.7(c)14(r)15.8(o)0(b)30.2(i)18.7(ol)-262(20)30.2(0)18.2(1)0(;)30.8(6)18.2(7:)30.8(12)30.2(2)18.2(5)0()]TJ 0 -1.1223 TD -.0182 Tc [(12)-18.2(3)12(1)0(.)]TJ -1.2503 -1.1284 TD 0 Tc [(9.)-500.4(Yanong)-439.7(RPE,)-430.9(Pouder)-437.2(DB,)-439.8(Falkinham)-438(JO.)-434.1(Association)-441.1(of)]TJ 1.2503 -1.1223 TD [(mycobacteria)-682.2(in)-682.8(recirculating)-679.8(aquaculture)-684.4(systems)-678.6(and)]TJ 0 -1.1284 TD [(mycobacterial)-358.6(disease)-358.9(in)-359.6(sh.)-350.2(J)-359.1(Aquat)-353.6(Anim)-361(Health)-357.4(2010;)]TJ 0 -1.1223 TD (22:219223.)Tj -1.7505 -1.1284 TD [(10.)-500.5(Francis-Floyd)-360.3(R:)-352.2(Mycobacterial)-364.3(Infections)-360.1(of)-350.3(Fish.)-355.8(South-)]TJ 1.7505 -1.1223 TD [(ern)-421.7(Regional)-428(Aquaculture)-425.6(Center.)-424.5(SRAC)-429.4(Publication)-425.4(No.)]TJ 0 -1.1284 TD [(4706,)-336.1(Southern)-333.3(Regional)-336.5(Aquaculture)-334.1(Center,)-339.1(2011.)]TJ -1.7505 -1.1223 TD [(11.)-500.5(Whipps)-540.5(CM,)-535(Lieggi)-542.6(C,)-541.7(Wagner,)-540.6(R.)-535.6(Mycobacteriosis)-547.7(in)]TJ 1.7505 -1.1284 TD [(zebrash)-335.3(colonies.)-337(ILAR)-334.4(J)-334.7(2012;53:95105.)]TJ -1.7505 -1.1223 TD .011 Tc [(1)11(2)11(.)-489.5(Ramsay)-269.7(JM,)-275.7(W).4(atral)-275.2(V)3.2(,)-275.7(S)1.9(chreck)-275.8(CB,)-275.7(Kent)-275.2(M)11(L)-8.7(.)-275.7(H)3.2(us)11(b)-6.7(a).6(ndry)]TJ 1.7505 -1.1284 TD .0107 Tc [(stress)-397.2(exacerbates)-397.2(m)-1.2(ycobac)-5.8(t)10.7(e)-5.4(ri)10.7(a)-5.4(l)-403.6(i)10.7(n)-7.3(fections)-397.2(in)-398.1(adult)-397.5(zeb-)]TJ 0 -1.1223 TD .0095 Tc [(ra)-5.7(s)9.5(h)-8.2(,)]TJ /F6 1 Tf 2.9276 0 TD .0113 Tc [(Dani)11.3(o)-403.2(r)-.1(erio)]TJ /F5 1 Tf 5.3917 0 TD .0121 Tc [(\(Hamilt)12.1(o)-5.9(n)5.9(\).)-396.6(J)-395.8(F)3(is)12.1(h)-402(D)-1.8(is)-395.8(2)5.9(0)-.2(0)5.9(9)-.2(;3)5.9(2:9)5.9(31)]TJ -8.3194 -1.1284 TD .0123 Tc [(94)6.1(1.)]TJ -1.7505 -1.1223 TD 0 Tc [(13.)-500.5(Whipps)-388(CM,)-388.7(Dougan)-384.8(ST,)-389.4(Kent)-383.9(ML.)]TJ /F6 1 Tf 17.2669 0 TD [(Mycobacterium)-392.8(hae-)]TJ -15.5165 -1.1284 TD (mophilum)Tj /F5 1 Tf 4.3244 0 TD [(infections)-326.7(of)-325.9(zebrash)-329.2(\()]TJ /F6 1 Tf 9.7161 0 TD [(Danio)-325(rerio)]TJ /F5 1 Tf 4.8245 0 TD [(\))-325.7(i)0(n)-323(research)]TJ -18.8649 -1.1223 TD [(facilities.)-336(FEMS)-336.9(Microbiol)-334.7(Lett)-334.9(2007;270:2126.)]TJ -1.7505 -1.1284 TD [(14.)-500.5(Mainous)-295.8(ME,)-294.3(Smith)-288.7(SA.)-297.6(Efcacy)-295.2(of)-289.3(common)-297(disinfectants)]TJ 1.7505 -1.1223 TD (against)Tj /F6 1 Tf 3.3729 0 TD [(Mycobacterium)-539.2(marinum)]TJ /F5 1 Tf 10.4297 0 TD [(.)-530.7(J)-536(Aquat)-530.5(Anim)-537.9(Health)]TJ -13.8026 -1.1284 TD (2005;17:284288.)Tj -1.7505 -1.1223 TD [(15.)-500.5(Best)-410.7(M,)-402(Sattar)-409.8(SA,)-407.4(Springthorpe)-408.8(VS,)-401.3(Kennedy)-413.6(ME.)-404.1(Ef-)]TJ 1.7505 -1.1284 TD [(cacies)-357.8(of)-356.4(selected)-357.5(disinfectants)-354.3(against)]TJ /F6 1 Tf 16.2118 0 TD [(Mycobacterium)-362.3(tu-)]TJ -16.2118 -1.1223 TD (berculosis)Tj /F5 1 Tf 4.117 0 TD [(.)-329.4(J)-334.7(Clin)-333.6(Microbiol)-334.7(1990;28:22342239.)]TJ -5.8675 -1.1284 TD [(16.)-500.5(Gauthier)-451.5(DT,)-443(Rhodes)-448.1(MW.)-449.2(Mycobacteriosis)-450.1(in)-445(shes:)-450.9(A)]TJ 1.7505 -1.1223 TD [(review.)-335.9(Vet)-335(J)-334.7(2009;180:3347.)]TJ -1.7505 -1.1284 TD [(17.)-500.5(Westereld)-387.6(M:)-389.3(The)-384.7(Zebrash)-387.8(Book.)-383.6(A)-386(Guide)-390.2(for)-383.2(the)-388.3(La-)]TJ 1.7505 -1.1223 TD [(boratory)-290(Use)-285.9(of)-283.2(Zebrash)-290.3(\()]TJ /F6 1 Tf 11.0396 0 TD [(Danio)-288.4(rerio)]TJ /F5 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